Differential gene transcription across the life cycle in Daphnia magna using a new all genome custom-made microarray

BMC Genomics
Bruno CamposCarlos Barata

Abstract

Unravelling the link between genes and environment across the life cycle is a challenging goal that requires model organisms with well-characterized life-cycles, ecological interactions in nature, tractability in the laboratory, and available genomic tools. Very few well-studied invertebrate model species meet these requirements, being the waterflea Daphnia magna one of them. Here we report a full genome transcription profiling of D. magna during its life-cycle. The study was performed using a new microarray platform designed from the complete set of gene models representing the whole transcribed genome of D. magna. Up to 93% of the existing 41,317 D. magna gene models showed differential transcription patterns across the developmental stages of D. magna, 59% of which were functionally annotated. Embryos showed the highest number of unique transcribed genes, mainly related to DNA, RNA, and ribosome biogenesis, likely related to cellular proliferation and morphogenesis of the several body organs. Adult females showed an enrichment of transcripts for genes involved in reproductive processes. These female-specific transcripts were essentially absent in males, whose transcriptome was enriched in specific genes of male sexual differ...Continue Reading

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Datasets Mentioned

BETA
GSE90810

Methods Mentioned

BETA
RAP
Feature Extraction

Software Mentioned

Acession
Gene Spring GX
BLASTX
Experiment viewer MeV4
Multi
Primer Express®
Agilent Feature Extraction
Agilent

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