Differential glycosaminoglycan binding of Chlamydia trachomatis OmcB protein from serovars E and LGV

Journal of Medical Microbiology
Sanaa Fadel, Adrian Eley

Abstract

We recently showed that OmcB protein from Chlamydia trachomatis serovar LGV1 functions as an adhesin. In this study, we produced Escherichia coli expressing OmcB from serovar E and compared this OmcB to OmcB from serovar LGV1. Infectivity inhibition assays carried out with serovars LGV1 and E of C. trachomatis in the presence of recombinant OmcB showed considerable (approximately 60%) inhibition of infectivity. In the presence of heparan sulphate, there was significant inhibition (68%) of adherence of E. coli expressing OmcB from serovar LGV1 only. In a further experiment, recombinant OmcB from serovar LGV1 showed minimal binding to glycosaminoglycan (GAG)-deficient cells, whilst to the same cells, recombinant OmcB from serovar E showed binding equal to that to the wild-type cells. Our experiments strongly suggest that OmcB from serovar E, in contrast to that from serovar LGV1, is not binding to host cells through a GAG-dependent mechanism.

References

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Jan 20, 2004·Journal of Medical Microbiology·Sanaa Fadel, Adrian Eley
Dec 19, 2006·Journal of Medical Microbiology·Sanaa Fadel, Adrian Eley

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Citations

Jun 8, 2012·Proceedings of the National Academy of Sciences of the United States of America·David M RosmarinHidde L Ploegh
Jul 10, 2012·Glycobiology·Vaibhav TiwariDeepak Shukla
Dec 12, 2012·Journal of Bacteriology·Shuping HouGuangming Zhong
Sep 24, 2013·Journal of Bacteriology·Tim FechtnerJohannes H Hegemann
Nov 9, 2012·Critical Reviews in Microbiology·Delphine S BeeckmanDaisy Vanrompay
Apr 5, 2013·Biological Reviews of the Cambridge Philosophical Society·Eyal KamhiRobert J Linhardt
Nov 26, 2015·Cellular Microbiology·Sonja Stallmann, Johannes H Hegemann
Mar 26, 2013·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Alexandra NunesJoão P Gomes
Nov 26, 2013·Cell Communication and Signaling : CCS·Adrian Mehlitz, Thomas Rudel
Jan 22, 2019·Critical Reviews in Microbiology·Signe ChristensenWilhelmina M Huston
Feb 26, 2020·Critical Reviews in Microbiology·Arlieke GitselsDaisy Vanrompay
Jun 13, 2018·Proceedings of the National Academy of Sciences of the United States of America·Agustin L LujanGabriel A Rabinovich
Oct 28, 2019·Frontiers in Microbiology·Arlieke GitselsDaisy Vanrompay
Oct 26, 2021·Journal of Basic Microbiology·Lin LiangDewen Tong

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