Differential growth of axons from sensory and motor neurons through a regenerative electrode: a stereological, retrograde tracer, and functional study in the rat

Neuroscience
P NegredoC Avendaño

Abstract

Polyimide regenerative electrodes (RE) constitute a promising neural interface to selectively stimulate regenerating fibers in injured nerves. The characteristics of the regeneration through an implanted RE, however, are only beginning to be established. It was recently shown that the number of myelinated fibers distal to the implant reached control values 7 months postimplant; however, the functional recovery remained substantially below normal [J Biomed Mater Res 60 (2002) 517]. In this study we sought to determine the magnitude, and possible selectivity, of axonal regeneration through the RE by counting sensory and motor neurons that were retrogradely labeled from double tracer deposits in the sciatic nerve. Adult rats had their right sciatic nerves transected, and the stumps were placed in silicone tubes; some simply were filled with saline (Tube group), and others held a RE in its center (RE group). Simultaneously, the proximal stump was exposed to Diamidino Yellow. Two months later the nerves were bilaterally excised distal to the implant, and exposed to Fast Blue. Electrophysiological recordings, and skin nociceptive responses confirmed previous findings of partial functional recovery. In controls, an average of 20,000 a...Continue Reading

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Citations

Feb 7, 2013·Plastic and Reconstructive Surgery·Jung-Hsien HsiehSung-Tsang Hsieh
Sep 16, 2015·The European Journal of Neuroscience·Akhil SrinivasanRavi V Bellamkonda
Jan 13, 2016·Medical & Biological Engineering & Computing·Anoop C Patil, Nitish V Thakor
Jan 26, 2016·Neural Regeneration Research·Krzysztof SuszyńskiDariusz Górka
Oct 14, 2005·Journal of the Peripheral Nervous System : JPNS·Xavier NavarroPaolo Dario
Jul 15, 2009·The Journal of Comparative Neurology·Ana M Lucas-Osma, Jorge E Collazos-Castro
Mar 29, 2014·Advanced Materials·Pouria FattahiMohammad Reza Abidian
Oct 4, 2012·IEEE Transactions on Neural Systems and Rehabilitation Engineering : a Publication of the IEEE Engineering in Medicine and Biology Society·Isaac P ClementsRavi V Bellamkonda
Jul 1, 2008·IEEE Transactions on Neural Systems and Rehabilitation Engineering : a Publication of the IEEE Engineering in Medicine and Biology Society·Fivos PanetsosVanessa Bonacasa
Jun 8, 2007·IEEE Transactions on Bio-medical Engineering·Natalia LagoXavier Navarro
Dec 15, 2010·IEEE Transactions on Neural Systems and Rehabilitation Engineering : a Publication of the IEEE Engineering in Medicine and Biology Society·Dan LewitusXiaofeng Jia
Jul 16, 2015·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Anna Puigdellívol-SánchezAlberto Prats-Galino
Dec 23, 2016·Muscle & Nerve·Maud CrezeThomas Bessede
Aug 7, 2018·The Journal of Comparative Neurology·Nuria García-MagroCarlos Avendaño
May 18, 2006·Journal of Neural Engineering·Anup RamachandranThomas Stieglitz

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