Differential IgE sensitization to cypress pollen associated to a basic allergen of 14 kDa

The FEBS Journal
Y ShahaliPascal Poncet

Abstract

The common cypress (Cupressus sempervirens) (Cups) pollen represents the first cause of respiratory allergies in the Mediterranean basin. The aim of this study was to characterize a novel 14-kDa cypress pollen allergen (BP14) allowing a clear dissociation of IgE sensitization patterns among allergic patients. The biochemical and immunochemical characterization of BP14 included determination of its isoelectric point, molecular mass, extraction kinetics, IgE binding prevalence, the presence of bromelain-type cross-reactive carbohydrate determinant and its IgE reactivity under reducing conditions. The presence of potential cross-reactive homologues in closely related cypress species, i.e. Cupressus arizonica (Cupa) and Cryptomeria japonica (Cryj), as well as in several taxonomically unrelated species was also investigated. According to our results, BP14 is easily and quickly solubilized in phosphate-buffered saline and exhibits several allergenic isoforms covering a broad range of pI (6.5-10.5). This allergen displays heat-stable conformational epitopes and does not include cross-reactive carbohydrate determinants, in contrast to high molecular weight cypress allergens. BP14 is expressed at higher levels in Cups than in Cupa and C...Continue Reading

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Citations

Oct 16, 2013·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Youcef ShahaliPascal Poncet
Jul 21, 2012·Journal of Proteomics·Youcef ShahaliPascal Poncet
Oct 30, 2016·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Angelica TiotiuPascal Poncet
Apr 13, 2017·Clinical Reviews in Allergy & Immunology·Denis CharpinPascal Poncet
May 17, 2019·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Pascal PoncetHélène Sénéchal
Jun 9, 2020·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Angelica E EhrenbergJonas Lidholm
Jul 22, 2020·Expert Review of Clinical Immunology·Pascal PoncetDenis Charpin
Jun 20, 2020·Clinical and Translational Allergy·Claudia AlessandriAdriano Mari
Jan 1, 2016·Allergy & Rhinology·Javier Domínguez-OrtegaJerónimo Carnés

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