Differential Immune Activating, Anti-Inflammatory, and Regenerative Properties of the Aqueous, Ethanol, and Solid Fractions of a Medicinal Mushroom Blend

Journal of Inflammation Research
Renee DavisGitte S Jensen

Abstract

To compare three fractions of a medicinal mushroom blend (MMB), MyCommunity, on immune-activation, inflammation-regulation, and induction of biomarkers involved in regenerative functions. A seventeen-species MMB was sequentially extracted: first, saline solution at ambient temperature, followed by re-extraction of the solids in ethanol, and finally resuspension of the homogenized ethanol-insoluble solids in cell-culture media. Fractions were tested on peripheral blood mononuclear cells from three healthy donors. Immunostaining, flow-cytometry, and Luminex protein-arrays measured immune-cell activation and cytokine response. Dose-responses for induction of the CD69 early activation marker and individual cytokine and growth-factor responses for each donor were evaluated. The CD69 and the combined cytokine and growth-factor results were subjected to Non-metric Multidimensional Scaling (NMDS) and multivariate ordination to aid interpretation of the aggregate immune response and pairwise permutational MANOVA on a distance-matrix to evaluate statistical differences between treatments on pooled data from all donors. Differential effects were induced by water-soluble, ethanol-soluble, and insoluble immunomodulatory compounds of the MMB...Continue Reading

References

May 23, 1998·Annual Review of Immunology·W P ArendC Gabay
Mar 7, 2000·Current Medicinal Chemistry·V E Ooi, F Liu
Feb 24, 2001·Bioscience, Biotechnology, and Biochemistry·E W LeeH Kawagish
Feb 21, 2004·International Immunopharmacology·Hong LinSusanna Cunningham Rundles
May 12, 2005·Journal of Agricultural and Food Chemistry·Tao WuCarl E Sams
Dec 4, 2008·The American Journal of Chinese Medicine·Xiaoxia ZhouClaudius U Meyer
Nov 9, 2010·Bioorganic & Medicinal Chemistry·Wan-Yu ChenDaniel Tzu-Bi Shih
Nov 13, 2012·Anti-cancer Agents in Medicinal Chemistry·Taek Joon YoonKwang Ho Lee
Dec 12, 2012·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Ann H RosendahlRoland Andersson
Jun 19, 2014·International Journal of Medicinal Mushrooms·Dong Hee Lee, Ha Won Kim
Mar 19, 2015·Integrative Cancer Therapies·Heidi FritzDugald Seely
Jul 2, 2015·International Journal of Immunopathology and Pharmacology·Jianping YangZhong Li
Nov 18, 2015·Carbohydrate Polymers·Andrea C RuthesMarcello Iacomini
Nov 18, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Courtney M JohnsonJonathan S Reichner
Apr 4, 2017·International Journal of Biological Macromolecules·Xirui HeZefeng Zhao
Apr 18, 2017·Stem Cell Research & Therapy·Elena Redondo-CastroEmmanuel Pinteaux
Jun 14, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Qiong WangZhongyang Ding

❮ Previous
Next ❯

Citations

Apr 4, 2021·Journal of Fungi·Yong-Sung ParkCheol-Won Yun

❮ Previous
Next ❯

Methods Mentioned

BETA
flow-cytometry

Software Mentioned

NMDS
vegan
RStudio
R
R ‘ stats
pgirmess
vegan3d
car
RVAideMemoire
dplyr

Related Concepts

Related Feeds

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.