Differential influence of the two Bacillus anthracis plasmids on regulation of virulence gene expression.

Infection and Immunity
A Fouet, M Mock

Abstract

Fully virulent Bacillus anthracis bacilli are encapsulated and toxinogenic. These bacteria contain two plasmids, pXO1 and pXO2, carrying genes coding for toxins (pag, lef, and cya) and for capsule synthetic enzymes (capB, capC, capA, and dep), respectively. A transcriptional fusion between the capB regulatory region and the lacZ reporter gene was constructed to study the regulation of capsule synthesis. A single copy of this fusion was inserted into the cap region of pXO2. The influence of environmental factors on the capB-lacZ fusion expression was initially analyzed in a pXO1-negative background: bicarbonate but not temperature induced the transcription from the capB promoter. A strain carrying the recombinant pXO2 and (delta)pag pXO1 was constructed for transregulatory studies. The pXO1 plasmid strongly enhanced capsule formation without modifying the bicarbonate-dependent induction level. A (delta)cap pXO2 was transduced into a strain containing pXO1 harboring a pag-lacZ transcriptional fusion (19). pXO2 showed no influence on the toxin gene transcription.

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Citations

Sep 7, 2001·Annual Review of Microbiology·M Mock, A Fouet
Apr 16, 2002·Molecular Microbiology·Tâm MignotAgnès Fouet
Dec 12, 2001·Molecular Microbiology·C Guidi-RontaniM Mock
Jan 25, 2003·Cellular Microbiology·Michèle Mock, Tâm Mignot
Nov 22, 2008·PLoS Pathogens·Adam C WilsonMarta Perego
Sep 20, 2011·Molecular Microbiology·Troy G HammerstromTheresa M Koehler
Sep 14, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Alice ChâteauAgnès Fouet
Jan 15, 2014·PloS One·Haim LevyDavid Kobiler
May 17, 2014·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Thomas CandelaIvo G Boneca
Oct 7, 2015·Frontiers in Microbiology·Muhammad Z ShabbirMasood Rabbani
May 1, 2004·Critical Reviews in Microbiology·Abanti ChattopadhyayRakesh Bhatnagar
Jun 5, 2007·PLoS Pathogens·Ian J GlomskiPierre L Goossens
May 24, 2005·Infection and Immunity·Janine M LamonicaVito G DelVecchio
Apr 22, 2003·Infection and Immunity·Agathe BourgogneTheresa M Koehler
Nov 6, 2018·The FEBS Journal·Sneha RamaswamyAnnalisa Pastore
May 6, 2005·Journal of Clinical Microbiology·Vincent PerretenJoachim Frey
Nov 1, 2002·Journal of Clinical Microbiology·Steven M LevineMartin J Blaser
Jul 21, 2021·MSystems·Yoshikazu FurutaHideaki Higashi
Jul 18, 2009·Molecular Aspects of Medicine·Agnès Fouet

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