Differential inhibition by allylsulfide of nitrification and methane oxidation in freshwater sediment.

Applied and Environmental Microbiology
R Roy, R Knowles

Abstract

Addition of nitrapyrin, allylthiourea, C(inf2)H(inf2), and CH(inf3)F to freshwater sediment slurries inhibited CH(inf4) oxidation and nitrification to similar extents. Dicyandiamide and allylsulfide were less inhibitory for CH(inf4) oxidation than for nitrification. Allylsulfide was the most potent inhibitor of nitrification, and the estimated 50% inhibitory concentrations for this process and CH(inf4) oxidation were 0.2 and 121 (mu)M, respectively. At a concentration of 2 (mu)M allylsulfide, growth and CH(inf4) oxidation activity of Methylosinus trichosporium OB3b were not inhibited. Allylsulfide at 200 (mu)M inhibited the growth of M. trichosporium by approximately 50% but did not inhibit CH(inf4) oxidation activity. Nitrite production by cells of M. trichosporium was not significantly affected by allylsulfide, except at a concentration of 2 mM, when growth and CH(inf4) oxidation were also inhibited by about 50%. Methane monooxygenase activity present in soluble fractions of M. trichosporium was not inhibited significantly by allylsulfide at either 200 (mu)M or 2 mM. These results suggest that the partial inhibition of CH(inf4) oxidation in sediment slurries by high allylsulfide concentrations may be caused by an inhibition o...Continue Reading

References

Jan 1, 1990·Methods in Enzymology·B G FoxJ D Lipscomb
Jan 1, 1990·Methods in Enzymology·J W WhittakerJ D Lipscomb
Jan 1, 1987·Annual Review of Biochemistry·D W Nebert, F J Gonzalez
Apr 1, 1993·Journal of Bacteriology·H McTavishA B Hooper
Sep 1, 1992·Applied and Environmental Microbiology·R S Oremland, C W Culbertson
Nov 1, 1993·Applied and Environmental Microbiology·L Y JulietteD J Arp
Nov 1, 1994·Applied and Environmental Microbiology·J A Amaral, R Knowles

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Citations

Sep 2, 2011·Environmental Technology·Simone VendramelGeraldo L Sant'Anna
Oct 1, 2002·FEMS Microbiology Ecology·Monique J M BieHendrikus J Laanbroek
Mar 15, 2006·Applied and Environmental Microbiology·F Van Der NatH J Laanbroek

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