Differential inhibitory effects of garlic-derived organosulfur compounds on cholesterol biosynthesis in primary rat hepatocyte cultures

Lipids
R Gebhardt, H Beck

Abstract

Using primary rat hepatocyte cultures, the potency of several garlic-derived organosulfur compounds to inhibit cholesterol biosynthesis in toto as well as at early and late steps of this metabolic pathway was compared. Concerning early steps, allicin significantly inhibited incorporation of [14C]acetate into nonsaponifiable neutral lipids already at concentrations as low as 10 microM, while diallyl disulfide and allyl mercaptan were effective above 100 microM only. Likewise, inhibition in response to the two vinyl-dithiins started at 500 microM. If [14C]acetate was replaced by [14C]mevalonate, inhibition due to allicin, diallyl disulfide, and allyl mercaptan disappeared suggesting that HMGCoA-reductase was the target of inhibition. In contrast, for the vinyl-dithiins a stimulation of mevalonate incorporation was found. Concerning the late step, the potency to exert accumulation of lanosterol presumably by inhibiting lanosterol 14 alpha-demethylase decreased in the order allicin > diallyl disulfide > allyl mercaptan = 1,3-vinyl-dithiin > 1,2-vinyldithiin, the effect of the latter compound being close to zero. With respect to the total inhibition of [14C]acetate labeling of cholesterol, the half-maximal effective concentration-va...Continue Reading

References

Oct 1, 1979·Computers and Biomedical Research, an International Journal·M C KohnD Garfinkel
Aug 1, 1992·Planta medica·C Egen-SchwindF H Kemper
Feb 1, 1992·Planta medica·C Egen-SchwindH Winterhoff
Mar 1, 1985·Scientific American·E Block
Jun 23, 1994·Biochimica Et Biophysica Acta·R GebhardtK G Wagner
Jun 1, 1993·The American Journal of Medicine·A K JainF G McMahon
May 1, 1993·The Clinical Investigator·H KiesewetterE Wenzel
Jul 1, 1995·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·R Gebhardt

❮ Previous
Next ❯

Citations

Jul 16, 2010·Cell Biochemistry and Biophysics·Ria R Ramoutar, Julia L Brumaghim
Mar 18, 2000·Journal of Ethnopharmacology·C V PantojaC M Contreras
Oct 29, 2000·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·B H Cho, S Xu
Jun 1, 2011·Nutrition Research Reviews·S V RanaR P Ola
Mar 22, 2002·Clinical and Experimental Pharmacology & Physiology·David D KuGary A Abrams
Dec 24, 2005·Critical Reviews in Food Science and Nutrition·N BhagyalakshmiR V Sreedhar
Dec 12, 2012·Critical Reviews in Food Science and Nutrition·Tao ZengKe-Qin Xie
May 4, 2013·Critical Reviews in Food Science and Nutrition·Lun LiPeng Zhang
May 1, 2013·Critical Reviews in Food Science and Nutrition·Anthony Fardet, Jean-Michel Chardigny
Oct 20, 2009·Journal of Ethnopharmacology·Bhavna SharmaPartha Roy
May 12, 2009·Chemico-biological Interactions·D TruongP J O'Brien
Mar 10, 2009·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·S A NasimA Mujib
Aug 21, 2008·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Mehmet ArhanIlker Durak
Dec 4, 2008·Phytotherapy Research : PTR·Suresh AmbatiClifton A Baile
Aug 14, 2012·Phytotherapy Research : PTR·Jackie Yan-Yan ChanShun-Wan Chan
May 26, 2004·The Journal of Nutritional Biochemistry·Ilker DurakHasan Serdar Oztürk
Jun 4, 2015·Journal of Endocrinological Investigation·A Hosseini, H Hosseinzadeh
Nov 7, 2006·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·A EidiE Esmaeili
Jul 17, 2015·Oxidative Medicine and Cellular Longevity·Swapna Upadhyay, Madhulika Dixit
Feb 15, 2011·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Esfandiar HeidarianAli Seidkhani-Nahal
Oct 14, 2010·Molecular Aspects of Medicine·Marcela A Vazquez-Prieto, Roberto M Miatello
Jan 23, 2003·Nutrition Journal·Sanjay K Banerjee, Subir K Maulik
Feb 12, 2015·Nutrition Research and Practice·Ae Wha HaWoo Kyoung Kim
May 23, 2007·Journal of Dairy Science·S CalsamigliaA Ferret
Nov 1, 2002·Phytotherapy Research : PTR·Gareth GriffithsBrian Smith
Nov 18, 2005·Journal of Dairy Science·M BusquetC Kamel
Jan 24, 2006·Journal of Dairy Science·M BusquetC Kamel
Dec 12, 2019·Critical Reviews in Food Science and Nutrition·Dinh-Chuong PhamBharath Kumar Velmurugan
Jun 6, 2020·Animal : an International Journal of Animal Bioscience·M R A RedoyM Al-Mamun
Jul 28, 2001·Nutrition Reviews·D Spigelski, P J Jones
Feb 21, 2003·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·E GermainC Teyssier

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.