Differential interactions between transforming growth factor-beta3/TbetaR1, TAB1, and CD2AP disrupt blood-testis barrier and Sertoli-germ cell adhesion

The Journal of Biological Chemistry
Weiliang XiaC Yan Cheng

Abstract

The biochemical basis that regulates the timely and selective opening of the blood-testis barrier (BTB) to migrating preleptotene/leptotene spermatocytes at stage VIII of the epithelial cycle in adult rat testes is virtually unknown. Recent studies have shown that cytokines (e.g. transforming growth factor (TGF)-beta3) may play a crucial role in this event. However, much of this information relies on the use of toxicants (e.g. CdCl(2)), making it difficult to relay these findings to normal testicular physiology. Here we report that overexpression of TGF-beta3 in primary Sertoli cells cultured in vitro indeed perturbed the tight junction (TJ) barrier with a concomitant decline in the production of BTB constituent proteins as follows: occludin, N-cadherin, and ZO-1. Additionally, local administration of TGF-beta3 to testes in vivo was shown to reversibly perturb the BTB integrity and Sertoli-germ cell adhesion via the p38 MAPK and ERK signaling pathways. Most importantly, the simultaneous activation of p38 and ERK signaling pathways is dependent on the association of the TGF-beta3-TbetaR1 complex with adaptors TAB1 and CD2AP because if TbetaR1 was associated preferentially with CD2AP, only Sertoli-germ cell adhesion was perturbed...Continue Reading

References

Dec 11, 1992·Nucleic Acids Research·H XuB T Liang
Jan 1, 1992·Microscopy Research and Technique·R M Pelletier, S W Byers
Feb 1, 1991·Endocrine Reviews·M K Skinner
Apr 19, 2000·Archives of Histology and Cytology·A W VoglJ Guttman
Mar 17, 2001·Nature Reviews. Molecular Cell Biology·J Massagué
Sep 22, 2001·Biology of Reproduction·M D AnwayM D Griswold
Jul 18, 2002·Current Biology : CB·Kumi Ozaki-KurodaYoshimi Takai
Dec 6, 2002·Endocrine Reviews·Hua ChangMartin M Matzuk
May 21, 2003·International Journal of Andrology·Wing-Yee LuiC Yan Cheng
Jun 18, 2003·Cell·Yigong Shi, Joan Massagué
Jan 22, 2004·Journal of Cell Science·Ching-Hang WongC Yan Cheng
Jun 24, 2004·The Journal of Biological Chemistry·Mario SchifferErwin P Böttinger
Nov 3, 2004·Trends in Endocrinology and Metabolism : TEM·Dolores D Mruk, C Yan Cheng
Jun 14, 2005·The Journal of Biological Chemistry·James A GrunkemeyerAndrey S Shaw
Jun 17, 2005·American Journal of Physiology. Cell Physiology·Yun FangIrena Levitan
Jul 19, 2005·Cytokine & Growth Factor Reviews·Weiliang XiaC Yan Cheng
Aug 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Helen H N Yan, C Yan Cheng
Dec 14, 2005·Current Topics in Developmental Biology·Ching-Hang Wong, C Yan Cheng

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Citations

Jul 12, 2011·Cellular and Molecular Life Sciences : CMLS·Pearl P Y LieDolores D Mruk
Jun 24, 2010·Nature Reviews. Endocrinology·C Yan Cheng, Dolores D Mruk
Jun 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Elissa W P WongC Yan Cheng
Dec 7, 2007·Biology of Reproduction·Oli SarkarDolores D Mruk
Jan 15, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Helen H N YanC Yan Cheng
Dec 31, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Pearl P Y LieDolores D Mruk
Oct 18, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Elissa W P WongC Yan Cheng
Apr 21, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Dolores D Mruk, C Y Cheng
May 17, 2008·Pharmacological Reviews·Dolores D MrukC Yan Cheng
Nov 1, 2011·Pharmacological Reviews·C Yan Cheng, Dolores D Mruk
Aug 10, 2012·American Journal of Physiology. Cell Physiology·Linlin SuC Yan Cheng
Feb 20, 2013·Journal of Molecular Endocrinology·Wenhui Su, Xinchun Liu
Aug 15, 2009·Journal of the American Society of Nephrology : JASN·Sandhya XavierErwin P Böttinger
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Weiliang XiaC Yan Cheng
Feb 18, 2011·Trends in Pharmacological Sciences·Elissa W P Wong, C Yan Cheng
Nov 4, 2010·Science China. Life Sciences·YiXun Liu, XiXia Li
Sep 16, 2008·Molecular and Cellular Endocrinology·Mushtaq A MemonMichael K Skinner
Oct 24, 2007·Biochimica Et Biophysica Acta·Lorenza González-MariscalDavid Chamorro
Aug 26, 2011·Spermatogenesis·C Yan ChengDolores D Mruk
Jan 24, 2013·Critical Reviews in Biochemistry and Molecular Biology·Wenhui SuC Yan Cheng
Feb 10, 2012·Spermatogenesis·C Yan Cheng, Dolores D Mruk
Jun 2, 2016·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Qian LiuQunwei Zhang
Oct 10, 2009·International Review of Cell and Molecular Biology·Elissa W P Wong, C Yan Cheng
Jan 18, 2013·Biology of Reproduction·Pawan Puri, William H Walker
Dec 26, 2006·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Helen H N YanC Yan Cheng
Feb 12, 2008·Journal of Cellular Physiology·A CatizoneM Galdieri
Jul 23, 2009·Critical Reviews in Biochemistry and Molecular Biology·C Yan Cheng, Dolores D Mruk
Mar 27, 2015·Cellular and Molecular Life Sciences : CMLS·Dongdong WangAndrei I Ivanov
Dec 19, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Nan LiC Yan Cheng
Jul 19, 2019·Cell Death & Disease·Fei-Da NiWan-Xi Yang
Sep 29, 2011·Molecular Biology Reports·Yun-Shu FanWan-Xi Yang
May 24, 2018·Environmental Science and Pollution Research International·Na ChenYa-Min Li
Nov 5, 2016·Basic & Clinical Pharmacology & Toxicology·Yuanchao CaoPeitao Wang

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