Differential involvement of Galpha16 in CC chemokine-induced stimulation of phospholipase Cbeta, ERK, and chemotaxis

Cellular Signalling
Yaji TianYung H Wong

Abstract

Chemokines are known to regulate the chemotaxis of leukocytes and play an important role in immunological processes. Chemokine receptors are widely distributed in hematopoietic cells and are often co-localized with the hematopoietic-specific G(16) and its close relative, G(14). Yet, many chemokine receptors utilize pertussis toxin-sensitive G(i) proteins for signaling. Given that both G(16) and G(14) are capable of linking G(i)-coupled receptors to the stimulation of phospholipase Cbeta, we examined the capacity of six CC chemokine receptors (CCR1, CCR2a, CCR2b, CCR3, CCR5 and CCR7) to interact with G(14) and G(16) in a heterologous expression system. Among the CC chemokine receptors tested, CCR1, CCR2b, and CCR3 were capable of mediating chemokine-induced stimulation of phospholipase Cbeta via either G(14) or G(16). The G(14)/G(16)-mediated responses exhibited CC chemokine dose-dependency and were resistant to pertussis toxin (PTX) treatment. In contrast, CCR2a, CCR5 and CCR7 were unable to interact with G(14) and G(16). Under identical experimental conditions, all six CC chemokine receptors were fully capable of inhibiting adenylyl cyclase via G(i) as well as stimulating phospholipase Cbeta via 16z44, a G(16/z) chimera that p...Continue Reading

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Citations

Feb 23, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·J Davin MillerLawrence S Prince
Oct 26, 2011·Future Medicinal Chemistry·Angela R Karash, Annette Gilchrist
Jan 1, 2009·Progress in Molecular Biology and Translational Science·Hyeseon Cho, John H Kehrl
Apr 30, 2019·Current Medicinal Chemistry·Frode SelheimAnna M Aragay
Nov 6, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Maggie M K LeeYung H Wong

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