Differential involvement of nuclear factor-kappaB and activator protein-1 pathways in the interleukin-1beta-mediated decrease of deiodinase type 1 and thyroid hormone receptor beta1 mRNA

The Journal of Endocrinology
J KwakkelA Boelen

Abstract

One of the hallmarks of the sick euthyroid syndrome or non-thyroidal illness is a decrease of serum triiodothyronine, caused mainly by a decrease in liver deiodinase type 1 (D1) mRNA and activity. Proinflammatory cytokines like interleukin (IL)-1beta are likely involved in this disease, but are also known to inhibit thyroid hormone receptor (TR)-beta1 gene expression, which is of interest as the D1 promoter contains TREs. The aim of the present study was to evaluate whether the IL-1beta-induced decrease of D1 and TRbeta1 mRNA is mediated by the same cytokine signalling pathways in a human hepatoma cell line (HepG2). We observed a downregulation of both D1 and TRbeta1 mRNA after 4 h of incubating the cells with IL-1beta. Sulfasalazine was used to inhibit the nuclear factor-kappaB (NFkappaB) pathway and SP600125, a chemical inhibitor of the c-Jun N-terminal kinase, was used as an inhibitor of the activator protein-1 (AP-1) pathway. AP-1 inhibition did not affect the decrease of D1 and TRbeta1 mRNA, but the TRbeta1 mRNA decrease was completely abolished after inhibiting NFkappaB, while D1 mRNA was unaffected. Only simultaneous inhibition of both the NFkappaB and AP-1 pathways abolished the D1 mRNA decrease. We concluded that IL-1b...Continue Reading

Citations

Oct 16, 2010·Cell and Tissue Research·Ihtzaz Ahmed MalikGiuliano Ramadori
May 10, 2008·Thyroid : Official Journal of the American Thyroid Association·Yves DebaveyeGreet Van den Berghe
Jun 19, 2007·Best Practice & Research. Clinical Endocrinology & Metabolism·Josef Köhrle
May 15, 2015·The Journal of Endocrinology·Emmely M de VriesAnita Boelen
Jul 26, 2019·Current Opinion in Endocrinology, Diabetes, and Obesity·An JacobsGreet Van den Berghe
Nov 21, 2019·Journal of the Endocrine Society·Lies LangoucheGreet Van den Berghe
Oct 23, 2010·European Journal of Clinical Investigation·Theodora A PappaMaria Alevizaki
Jul 16, 2019·Thyroid : Official Journal of the American Thyroid Association·Emmely M de VriesAnita Boelen
Sep 14, 2017·Thyroid : Official Journal of the American Thyroid Association·Takashi Akamizu
Nov 26, 2019·Endocrine, Metabolic & Immune Disorders Drug Targets·Khalid SiddiquiMuhammad Mujammami
Dec 16, 2020·Journal of Endocrinological Investigation·E Fliers, A Boelen
Feb 10, 2021·Journal of Endocrinological Investigation·L CroceL Chiovato
Mar 14, 2021·The Journal of Clinical Endocrinology and Metabolism·Inge GrondmanRomana T Netea-Maier

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.