PMID: 7526082Jun 1, 1994Paper

Differential labeling of converging afferent pathways using biotinylated dextran amine and cholera toxin subunit B

Journal of Neuroscience Methods
J M Alisky, D L Tolbert

Abstract

We report a new technique for 2-tracer anterograde labeling that permits unequivocal identification of the differentially labeled projections in the same section. One pathway is labeled with biotinylated dextran amine and is visualized as a black to dark gray diaminobenzidine (DAB)-cobalt precipitate by an avidin-biotinylated peroxidase reaction. The other pathway is labeled with cholera toxin subunit B and is visualized as a reddish-brown reaction product using DAB without cobalt as the substrate for peroxidase immunohistochemistry. To maintain serial order, sections can be processed mounted on slides without any loss of sensitivity for either tracer.

References

Oct 1, 1992·Journal of Neuroscience Methods·H M Brandt, A V Apkarian
Mar 1, 1992·Journal of Neuroscience Methods·C L VeenmanM G Honig
Aug 1, 1991·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R J Weinberg, S L van Eyck
Mar 1, 1984·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M B Hancock

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Citations

Nov 12, 1998·The Anatomical Record·H J Ralston
Nov 14, 2000·Journal of Neuroscience Methods·A ReinerM G Honig
Jul 20, 2002·Spine·Brian K KwonWolfram Tetzlaff
Feb 16, 2008·Neuroscience Letters·Nicole ChabotGilles Bronchti
May 3, 2006·Neuroscience Letters·Joseph Martin AliskyBeverly L Davidson

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