Differential labeling with orotic acid and uridine in compensatroy renal hypertrophy.

The American Journal of Physiology
J S RossR A Malt

Abstract

Using [5-3H]orotic acid and [5-3H]uridine as precursors, we compared the efficiency of labeling and the localization of labeled RNA during compensatory hypertrophy of the mouse kidney. [5-3H]orotic acid in tubules labeled RNA 15 times more intensely that [5-3H]uridine, presumably because of greater incorporation of orotic acid into tubular cells. Of the orotic acid label, 97% was in tubular cells, mostly in the proximal tubules. Only about 80% of the uridine label was in the tubules; the ratio in proximal tubules compared with that in distal tubules was 2:1. No changes in distribution within the nephron were produced during compensatory hypertrophy. [5-3H]uridine should be used as the precursor of generalized labeling is desired, but [5-3H5orotic acid is the better precursor of RNA for many studies of compensatory hypertrophy since it is more efficient and concentrates in the segments of greatest biologic activity.

Citations

Dec 16, 1998·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·K C ObergE P Hawkins
Feb 26, 2011·The Journal of Physiological Sciences : JPS·Daisaku MiuraHitoshi Endou
May 1, 1988·Molecular and Cellular Biology·G Watson, K Paigen
Jan 2, 1990·Molecular and Cellular Endocrinology·G Watson, K Paigen
Jul 27, 1976·Biochemistry·A J Ouellett, R A Malt
Apr 1, 1983·Kidney International·R A Malt

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