Differential levels of Neurod establish zebrafish endocrine pancreas cell fates.

Developmental Biology
Gokhan Dalgin, Victoria E Prince

Abstract

During development a network of transcription factors functions to differentiate foregut cells into pancreatic endocrine cells. Differentiation of appropriate numbers of each hormone-expressing endocrine cell type is essential for the normal development of the pancreas and ultimately for effective maintenance of blood glucose levels. A fuller understanding of the details of endocrine cell differentiation may contribute to development of cell replacement therapies to treat diabetes. In this study, by using morpholino and gRNA/Cas9 mediated knockdown we establish that differential levels of the basic-helix loop helix (bHLH) transcription factor Neurod are required for the differentiation of distinct endocrine cell types in developing zebrafish. While Neurod plays a role in the differentiation of all endocrine cells, we find that differentiation of glucagon-expressing alpha cells is disrupted by a minor reduction in Neurod levels, whereas differentiation of insulin-expressing beta cells is less sensitive to Neurod depletion. The endocrine cells that arise during embryonic stages to produce the primary islet, and those that arise subsequently during larval stages from the intra-pancreatic duct (IPD) to ultimately contribute to the ...Continue Reading

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Citations

Mar 11, 2016·Developmental Biology·Rebecca L BeerMeritxell Rovira
Apr 27, 2016·Scientific Reports·Surjya Narayan DashSanna Lehtonen
Feb 12, 2017·Frontiers in Endocrinology·Lisette A Maddison, Wenbiao Chen
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Sep 5, 2018·Frontiers in Cell and Developmental Biology·Liqing ZangWenbiao Chen
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Dec 9, 2020·Developmental Biology·Gökhan Dalgin, Victoria E Prince
Jan 16, 2021·Journal of Diabetes Investigation·Lingling YangRonald Cw Ma
Feb 6, 2019·Developmental Biology·Kamil AhsanVictoria E Prince

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