Differential lineage-specific regulation of murine CD45 transcription by Oct-1 and PU.1

Biochemical and Biophysical Research Communications
Un K KwonRichard A Wells

Abstract

Although it has been established that CD45 expression is regulated at the transcriptional level, neither the regulatory elements that are responsible for its unique expression pattern nor the relevance of its three distinct transcriptional start sites (P1a, P1b, and P2) has been fully characterized. We studied the contribution of the three start sites to CD45 mRNA production in haematopoietic cell lines and primary haematopoietic cells. In myeloid and lymphoid cells and cell lines most CD45 transcripts originate from P1b with the exception of the thymoma-derived T cell line EL4, in which approximately 90% of CD45 transcripts originate from P1a. The degree of contribution of P1a is highest in lymphoid cells and increases in T cells following mitogen stimulation. In vitro evaluation of sequence upstream of the start sites shows that the P2 start site is sufficient for CD45 expression in lymphoid but not in myeloid cells, confirms the presence of a PU.1-binding site essential for myeloid expression of CD45, and reveals an Octamer-binding site that interacts with both Oct-1 and Oct-2 and activates CD45 transcription in lymphoid and myeloid cells. These findings are the first evidence that Octamer-binding factors are involved in the...Continue Reading

References

Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·Y SagaE A Boyse
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·W C Raschke
Nov 1, 1988·Molecular and Cellular Biology·Y SagaE A Boyse
Jan 13, 1995·The Journal of Biological Chemistry·H SchwarzenbachP Matthias
Aug 1, 1994·International Immunology·J F DiMartinoJ S Lee
Jan 1, 1996·The Journal of Experimental Medicine·X MaG Trinchieri
Jun 28, 1996·The Journal of Biological Chemistry·H ChenD G Tenen
Feb 29, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·C S MaloneR Wall
Sep 2, 2000·Immunogenetics·V TovarP Engel
Sep 23, 2000·Molecular Immunology·C S MaloneR Wall
Dec 15, 2000·The Journal of Biological Chemistry·K L AndersonB E Torbett
Jun 5, 2001·The Journal of Biological Chemistry·E L Virts, W C Raschke

❮ Previous
Next ❯

Citations

Apr 5, 2011·Cellular and Molecular Life Sciences : CMLS·Fumiaki UchiumiSei-ichi Tanuma
Oct 16, 2010·The Journal of Biological Chemistry·Ryan C ThompsonThomas D Gilmore
Dec 2, 2011·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Fumiaki UchiumiSei-Ichi Tanuma

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.