Differential long noncoding RNAs expression in cancer-associated fibroblasts of non-small-cell lung cancer

Pharmacogenomics
Cuifang TengJie Yang

Abstract

The aim of this study was to investigate the role of long noncoding RNAs (lncRNAs) profiles in cancer-associated fibroblasts (CAFs) during non-small-cell lung cancer (NSCLC) progression. Differentially expressed lncRNAs and mRNAs were detected by lncRNA microarray between three patient-paired CAFs and the adjacent normal fibroblasts, which were obtained from tumoral and nontumoral portions of surgically resected lung tissue from three primary NSCLCs. Bioinformatic analyses including gene ontology and pathway analysis were applied to these differentially expressed mRNAs. The qRT-PCR was conducted to identify the change of selected lncRNAs that might be involved in contribution of CAFs toward NSCLC. A total of 766 lncRNAs and 750 mRNAs abnormally expressed in CAFs (fold-change >2, p < 0.05). Bioinformatic analyses indicated that these mRNAs are associated with immune function. The qPCR results were consistent with microarray data. The lncRNAs profiles of CAFs may provide promising targets for further research on immune regulation during NSCLC process.

References

Sep 27, 2007·Journal of the National Cancer Institute·Olivier TrédanIan F Tannock
Apr 9, 2011·Proceedings of the National Academy of Sciences of the United States of America·Roya NavabMing-Sound Tsao
Nov 19, 2011·Nature Reviews. Genetics·Manel Esteller
Nov 19, 2011·Cancer Discovery·John R Prensner, Arul M Chinnaiyan
Mar 3, 2012·PLoS Computational Biology·Purvesh KhatriAtul J Butte
Mar 23, 2013·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Christoph Kahlert, Raghu Kalluri
May 11, 2013·British Journal of Cancer·S W CheethamM E Dinger
Oct 10, 2014·Journal of Cellular and Molecular Medicine·Jing ChenLong-Bang Chen
Nov 21, 2014·British Journal of Cancer·L ShiC Zhao
Nov 28, 2014·Nucleic Acids Research·UNKNOWN Gene Ontology Consortium
Apr 22, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Haiwei SangMin Zhu
Jan 10, 2018·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal

❮ Previous
Next ❯

Citations

Nov 17, 2020·Frontiers in Oncology·Minjie WuAifu Lin
Nov 10, 2020·Life Sciences·Md Nazim Uddin, Xiaosheng Wang

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

SPSS
Arraystar
Ensembl
Rosetta Resolver System

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.