Differential mechanism for the cell surface sorting and agonist-promoted internalization of the alpha1B-adrenoceptor

British Journal of Pharmacology
A HirasawaG Tsujimoto

Abstract

1. Alpha1B-adrenoceptors are localized at a steady state in the plasma membrane in untreated cells, and internalize to intracellular vesicles when exposed to agonist. Flow cytometry analysis with an anti-N-terminus-antibody (1B-N1-C, (Hirasawa et al., 1996)) facilitated the quantification of cell surface alpha1B-adrenoceptor. Also, the cellular distribution of alpha1B-adrenoceptors was visually monitored by immunocytochemical confocal microscopy. 2. Utilizing this combined approach, we have examined the molecular mechanism for cellular trafficking of alpha1B-adrenoceptors, including the process of sorting of the synthesized receptor protein to the cell surface, and the agonist-induced internalization. The two processes were separately examined by using alpha1B-adrenoceptor inducible DDT1MF-2 cells for the sorting process and CHO cells stably expressing alpha1B-adrenoceptors for the agonist-promoted internalization. 3. We examined the effects of cytochalasin D and mycalolide B (actin depolymerization agents), demecolcine (a microtubule disrupting agent), brefeldin A (an inhibitor of vesicular transport and Golgi function), bafilomycin A1 (a specific inhibitor of the vacuolar proton pump) or hyperosmotic sucrose treatment (that m...Continue Reading

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Citations

May 15, 2007·Naunyn-Schmiedeberg's Archives of Pharmacology·Maikel JongsmaAstrid E Alewijnse
Sep 6, 2000·Proceedings. Biological Sciences·J Smythies
Dec 21, 2006·Biochemical and Biophysical Research Communications·Zhang-Yi LiangXin-Sheng Zhao
May 9, 2002·Biological & Pharmaceutical Bulletin·Taka-aki KoshimizuGozoh Tsujimoto
Jan 29, 2002·Journal of Cardiovascular Pharmacology·G TsujimotoA Tanoue
Feb 8, 2006·The Journal of Biological Chemistry·Zoya M VolovykHoward A Rockman
Jan 6, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·A ZaslaverA Ben-Baruch
Apr 23, 2004·American Journal of Physiology. Endocrinology and Metabolism·Hesham A W Tawfeek, Abdul B Abou-Samra
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Dec 3, 2008·The Journal of Biological Chemistry·Jonathan Barroso-GonzálezAgustín Valenzuela-Fernández
Dec 15, 2005·The Journal of Biological Chemistry·Laura StanasilaSusanna Cotecchia
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Jun 13, 2003·European Journal of Cell Biology·Tuire Olli-LähdesmäkiJaana Kallio
Dec 5, 2006·Biochemical Pharmacology·Peter Hein, Martin C Michel

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