Differential Metabolic Pathway Analysis of the Proteomes of Leishmania donovani and Leptomonas seymouri

Proteomics. Clinical Applications
Neeloo SinghEkta Jain

Abstract

Although in trypanosomatids, monoxeny (Leptomonas) is ancestral to dixeny (Leishmania), however clinical cases of visceral leishmanisis with Leptomonas co-infection are increasingly being reported from India. Using a proteogenomic approach, a detailed proteome analysis of these two kinetoplastid parasites viz., Leishmania and its sister Leptomonas, to catalog the key proteins associated with and therefore possibly responsible for phenotype changes in Leptomonas evolution and domestication as co-infection with Leishmania is carried out. LC-MS/MS is utilized for this proteomic purpose. One Leishmania donovani WHO reference strain and two Leptomonas seymouri isolates, which are originally isolated from clinical cases of kala azar patients with different inherent drug sensitivity viz., responsive and unresponsive, are used in this study. A network analysis, leveraging protein-protein interaction data helped to find the roles of the proteins in carbon metabolism and biosynthesis of secondary metabolites which is seen to be altered under stress conditions like drug resistance. The information provided about the metabolic pathways modulated when contrasting these two phenotypes may lead to the development of new strategies to block pa...Continue Reading

References

Oct 1, 1987·Molecular and Biochemical Parasitology·N Samaras, T W Spithill
Sep 30, 1999·Parasitology·I L MauricioM A Miles
Jan 5, 2002·Medical Microbiology and Immunology·M ShapiraV Yavesky
Aug 5, 2003·The Journal of Infectious Diseases·Neeloo SinghShyam Sundar
Nov 14, 2003·Kinetoplastid Biology and Disease·Véronique HannaertPaul AM Michels
Sep 25, 2004·International Journal of Systematic and Evolutionary Microbiology·David MoreiraKeith Vickerman
May 8, 2007·International Journal for Parasitology·Miguel A MoralesGerald F Späth
Jun 6, 2007·International Journal for Parasitology·Martin Wiese
Sep 4, 2007·Molecular and Biochemical Parasitology·Christine Clayton, Michal Shapira
Sep 22, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Doron RosenzweigDan Zilberstein
Jan 19, 2008·Proteomics·Miguel A MoralesGerald F Späth
May 14, 2008·Molecular & Cellular Proteomics : MCP·Daniel PaapeToni Aebischer
Oct 23, 2008·Nucleic Acids Research·Lars J JensenChristian von Mering
Aug 15, 2009·Interdisciplinary Perspectives on Infectious Diseases·Wanderley de Souza, Juliany Cola Fernandes Rodrigues
Sep 15, 2009·Trends in Parasitology·Aloysius G M Tielens, Jaap J van Hellemond
Feb 18, 2010·Parasitology·Eleanor C SaundersMalcolm J McConville
Jun 30, 2010·Journal of Proteome Research·Marie-Christine BrothertonMarc Ouellette
Jul 17, 2010·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Pankaj SrivastavaShyam Sundar
Oct 19, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·T LahavP J Myler
Mar 16, 2011·Current Topics in Medicinal Chemistry·Paul G WyattAlan H Fairlamb
Dec 30, 2011·PLoS Neglected Tropical Diseases·Ana Marta SilvaGraham H Coombs
May 25, 2012·Journal of Clinical Microbiology·Susmita GhoshMitali Chatterjee
Jun 14, 2012·PloS One·Jorge AlvarUNKNOWN WHO Leishmaniasis Control Team
Aug 29, 2012·PLoS Neglected Tropical Diseases·Imen RabhiUNKNOWN Sysco Consortium
Dec 4, 2012·Nucleic Acids Research·Andrea FranceschiniLars J Jensen
Aug 6, 2013·Frontiers in Plant Science·Kenneth M Olsen, Jonathan F Wendel
Dec 7, 2013·PloS One·Marie-Christine BrothertonMarc Ouellette
Jul 25, 2014·International Journal for Parasitology. Drugs and Drug Resistance·Marie-Christine BrothertonMarc Ouellette
Sep 11, 2014·The Journal of Investigative Dermatology·Claire da Silva SantosSerhiy Souchelnytskyi
Oct 10, 2015·Trends in Genetics : TIG·Carlos A BuscagliaFernán Agüero
Oct 24, 2015·Parasitology·Angamuthu SelvapandiyanAnuja Krishnan

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Jun 25, 2020·Expert Review of Proteomics·Khushman TaunkSrikanth Rapole

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