Differential migration, LPS-induced cytokine, chemokine, and NO expression in immortalized BV-2 and HAPI cell lines and primary microglial cultures.

Journal of Neurochemistry
Ryan HorvathJoyce A DeLeo

Abstract

Microglial cells are hematopoietically derived monocytes of the CNS and serve important neuromodulatory, neurotrophic, and neuroimmune roles. Following insult to the CNS, microglia develop a reactive phenotype, migrate to the site of injury, proliferate, and release a range of proinflammatory, anti-inflammatory, and neurotrophic factors. Isolation of primary microglial cell cultures has been an integral step in elucidating the many roles of these cells. In addition to primary microglial cells, several immortalized cell lines have been created to model primary microglia in vitro, including murine-derived BV-2 cells and rat derived highly aggressive proliferating immortalized (HAPI) cells. Here, we compare rat primary microglial, BV-2, and HAPI cells in experiments assessing migration, expression of activation markers, and production and release of nitric oxide, cytokines, and chemokines. BV-2 and HAPI cells responded similarly to primary microglia in experiments assessing migration, ionized calcium binding adaptor molecule 1 expression, and nitric oxide release. However, BV-2 and HAPI cells did not model primary microglia in experiments assessing tumor necrosis factor-alpha, interleukin-1beta, interleukin-6, and monocyte chemoat...Continue Reading

References

May 1, 1990·Journal of Neuroimmunology·E BlasiF Bistoni
Apr 30, 1999·Progress in Neurobiology·W J StreitN A Pennell
Aug 2, 2001·Journal of Biochemistry·K Nakajima, S Kohsaka
Jul 6, 2002·Journal of Neuroimmunology·Bei Ping HeMichael J Strong
Mar 17, 2004·Journal of Neuroimmunology·Sergey G KremlevCharles Palmer
Oct 16, 2004·International Clinical Psychopharmacology·Raphael M BonelliHans-Peter Kapfhammer
Dec 2, 2004·Neurobiology of Disease·David BlumMarc Levivier
Apr 19, 2005·Journal of Neuroimmunology·Sergey G Kremlev, Charles Palmer
Jun 28, 2005·Journal of Neuroimmunology·Vielska M BrautigamJyoti J Watters
Mar 16, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Giulio G MuccioliNephi Stella
Sep 18, 2007·Journal of Neurochemistry·Nancy Nutile-McMenemyJoyce A Deleo
Oct 30, 2007·Nature Neuroscience·Uwe-Karsten Hanisch, Helmut Kettenmann
Nov 6, 2007·Lancet Neurology·Paul H GordonUNKNOWN Western ALS Study Group
Jan 23, 2008·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Susanna ZierlerHubert H Kerschbaum
Feb 6, 2008·Journal of Neurochemistry·Eiko K de JongKnut Biber
Jun 4, 2008·Clinical Neuropharmacology·UNKNOWN NINDS NET-PD Investigators

❮ Previous
Next ❯

Citations

Dec 17, 2009·Acta Neuropathologica·Manuel B Graeber, Wolfgang J Streit
Feb 26, 2011·Pflügers Archiv : European journal of physiology·Jimmy Van den EyndenJean-Michel Rigo
Jul 8, 2011·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Daniel FragaGuy A Cabral
Oct 12, 2012·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Dawn BéraudKathleen A Maguire-Zeiss
Aug 4, 2012·ACS Chemical Neuroscience·Geeta S ParanjapeMichael R Nichols
Nov 16, 2011·The Journal of Experimental Medicine·Vivek SwarupJean-Pierre Julien
Jun 2, 2012·Journal of Neuroinflammation·Branden StansleyKenneth Hensley
Jun 2, 2012·Journal of Neuroinflammation·Hiromi KumamaruSeiji Okada
Jun 1, 2012·PloS One·Valerie L JacobsJoyce A De Leo
Jan 30, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ryan J Horvath, Joyce A DeLeo
Nov 14, 2013·Journal of Molecular Neuroscience : MN·Jian TuDeMing Zhao
Mar 13, 2014·Biochimica Et Biophysica Acta·Mian ZhouPing Wang
Apr 25, 2012·Acta Neuropathologica·Katja SchefflerJens Pahnke
Dec 4, 2010·Neurochemical Research·George H De Vries, Anne I Boullerne
Feb 27, 2013·Current Translational Geriatrics and Experimental Gerontology Reports·Tarja MalmJari Koistinaho
Jan 29, 2016·Journal of Neuroinflammation·Ryan C McCarthyMarianne Wessling-Resnick
Nov 27, 2015·Toxicological Sciences : an Official Journal of the Society of Toxicology·Alejandro M S MayerPhillip Williams
Nov 13, 2009·Journal of Neuroscience Research·Aroa Ejarque-OrtizJosep Saura
Jan 10, 2016·Neurotoxicity Research·Flávia V Santa-CecíliaRita Raisman-Vozari
Nov 13, 2015·Biomedical Microdevices·Kirsten H DodsonJon F Edd
May 21, 2009·Glia·Isabella NapoliHarald Neumann
Jun 15, 2010·Glia·Hwan-Suck ChungHyunsu Bae

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.