Differential modulation of GABAA and NMDA receptors by α7-nicotinic receptor desensitization in cultured rat hippocampal neurons

Acta Pharmacologica Sinica
Lei ShenHai Wang

Abstract

To explore the modulatory effect of desensitized α7-containing nicotinic receptors (α7-nAChRs) on excitatory and inhibitory amino acid receptors in cultured hippocampal neurons and to identify the mechanism underlying this effect. Whole-cell patch-clamp recordings were performed on cultured rat hippocampal neurons to measure α7-nAChR currents and to determine the role of desensitized α7-nAChRs on brain amino acid receptor activity. Pulse and perfusion applications of the α7-nAChR agonist choline were applied to induce different types of α7-nAChR desensitization in cultured hippocampal neurons. After a brief choline pulse, α7-nAChR was desensitized as a result of receptor activation, which reduced the response of the A type γ-aminobutyric acid (GABAA) receptor to its agonist, muscimol, and enhanced the response of the NMDA receptor to its agonist NMDA. By contrast, the responses of glycine or AMPA receptors to their agonists, glycine or AMPA, respectively, were not affected. Pretreatment with the α7-nAChR antagonist methyllycaconitine (MLA, 10 nmol/L) blocked the choline-induced negative modulation of the GABAA receptor and the positive modulation of the NMDA receptor. The regulation of the GABAA and NMDA receptors was confirmed...Continue Reading

References

May 10, 1995·Annals of the New York Academy of Sciences·R J Lukas
Sep 18, 1999·Science·R C Malenka, R A Nicoll
Apr 20, 2000·European Journal of Pharmacology·M AlkondonE X Albuquerque
Mar 4, 2003·Journal of Neurophysiology·Vladimir V UteshevRoger L Papke
May 26, 2005·Brain Research. Brain Research Reviews·Hai Wang, Xiulan Sun
Jun 28, 2005·Trends in Neurosciences·Rashid GiniatullinJerrel L Yakel
May 3, 2006·European Neuropsychopharmacology : the Journal of the European College of Neuropsychopharmacology·Jared W YoungJohn Sharkey
May 13, 2009·Acta Pharmacologica Sinica·Andon Nicholas PlaczekJohn Anthony Dani
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Manuela MazzuferiMichele Simonato
Sep 16, 2010·Nature Reviews. Neuroscience·Giles E Hardingham, Hilmar Bading

❮ Previous
Next ❯

Citations

Feb 7, 2018·Journal of Receptor and Signal Transduction Research·W R Williams
Jun 24, 2016·CNS Neuroscience & Therapeutics·Shu-Na WangChao-Yu Miao
Jun 18, 2017·Frontiers in Immunology·Fabiana Maria das Graças Corsi-ZuelliCamila Marcelino Loureiro
May 22, 2021·Current Opinion in Pharmacology·Frédéric Knoflach, Daniel Bertrand

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.