PMID: 8594875Dec 1, 1995Paper

Differential mRNA expression of insulin-like growth factor system during renal injury and hypertrophy

The American Journal of Physiology
M K HiseR M Rohan

Abstract

The cellular effects of insulin-like growth factor I (IGF-I) are modified by a family of binding proteins (IGFBPs) that act as reservoirs in serum for the growth factor and are produced locally by tissues, including the kidney. Because regulation of these proteins may influence renal repair, either directly or by their interactions with IGF-I, we studied gene expression during the recovery from renal failure induced by folic acid and during the compensatory increase in renal function following uninephrectomy (UNX). Expression of IGF-I, the IGF-I receptor (IGF-IR), and all six IGFBPs was detected using an ribonuclease protection assay. IGFBP-5 was the most abundant binding protein mRNA present in kidney, whereas IGFBP-2 and -6 were the least abundant. During regeneration following folic acid-induced acute renal failure, IGF-I, IGFBP-3, and IGFBP-5 mRNAs declined in abundance approximately two- to threefold. On the other hand, IGF-IR, IGFBP-1, and IGFBP-2 were increased (approximately 2-, 6-, and 6-fold, respectively) in the first 24 h. IGFBP-1 mRNA remained elevated for at least 3 days. Despite the known increase in cellular RNA content following UNX, little difference in specific expression of mRNAs was observed. Because IGFBP-...Continue Reading

Citations

Sep 1, 2000·American Journal of Physiology. Renal Physiology·E HuD Brooks
Jul 15, 2000·American Journal of Physiology. Renal Physiology·e S Nigam, W Lieberthal
Apr 9, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·G A BrockmannA Hoeflich

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