Differential Muc2 and Muc5ac secretion by stimulated guinea pig tracheal epithelial cells in vitro

Respiratory Research
B ChorleyLinda D Martin

Abstract

Mucus overproduction is a characteristic of inflammatory pulmonary diseases including asthma, chronic bronchitis, and cystic fibrosis. Expression of two mucin genes, MUC2 and MUC5AC, and their protein products (mucins), is modulated in certain disease states. Understanding the signaling mechanisms that regulate the production and secretion of these major mucus components may contribute significantly to development of effective therapies to modify their expression in inflamed airways. To study the differential expression of Muc2 and Muc5ac, a novel monoclonal antibody recognizing guinea pig Muc2 and a commercially-available antibody against human MUC5AC were optimized for recognition of specific guinea pig mucins by enzyme-linked immunosorbent assay (ELISA), Western blot, and immunohistochemistry (IHC). These antibodies were then used to analyze expression of Muc2 and another mucin subtype (likely Muc5ac) in guinea pig tracheal epithelial (GPTE) cells stimulated with a mixture of pro-inflammatory cytokines [tumor necrosis factor-alpha (TNF-alpha), interleukin 1beta (IL-1beta), and interferon- gamma (IFN-gamma)]. The anti-Muc2 (C4) and anti-MUC5AC (45M1) monoclonal antibodies specifically recognized proteins located in Muc2-domin...Continue Reading

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Citations

May 3, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Dongfang YuYin Chen
Oct 26, 2018·American Journal of Physiology. Lung Cellular and Molecular Physiology·Veronika E WinkelmannManfred Frick
Aug 27, 2011·American Journal of Respiratory Cell and Molecular Biology·Claudia KaracsonyiElliott Kagan
Dec 7, 2010·Expert Review of Respiratory Medicine·Joanne L Wright, Andrew Churg
Dec 22, 2019·International Journal of Molecular Sciences·Xin MengHao Wu

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Methods Mentioned

BETA
electrophoresis
ELISA
PCR
ELISAs

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