Differential NDR/LATS interactions with the human MOB family reveal a negative role for human MOB2 in the regulation of human NDR kinases.

Molecular and Cellular Biology
Reto S KohlerAlexander Hergovich

Abstract

MOB proteins are integral components of signaling pathways controlling important cellular processes, such as mitotic exit, centrosome duplication, apoptosis, and cell proliferation in eukaryotes. The human MOB protein family consists of six distinct members (human MOB1A [hMOB1A], -1B, -2, -3A, -3B, and -3C), with hMOB1A/B the best studied due to their putative tumor-suppressive functions through the regulation of NDR/LATS kinases. The roles of the other MOB proteins are less well defined. Accordingly, we characterized all six human MOB proteins in the context of NDR/LATS binding and their abilities to activate NDR/LATS kinases. hMOB3A/B/C proteins neither bind nor activate any of the four human NDR/LATS kinases. We found that both hMOB2 and hMOB1A bound to the N-terminal region of NDR1. However, our data suggest that the binding modes differ significantly. Our work revealed that hMOB2 competes with hMOB1A for NDR binding. hMOB2, in contrast to hMOB1A/B, is bound to unphosphorylated NDR. Moreover, RNA interference (RNAi) depletion of hMOB2 resulted in increased NDR kinase activity. Consistent with these findings, hMOB2 overexpression interfered with the functional roles of NDR in death receptor signaling and centrosome overdupli...Continue Reading

References

Mar 14, 1998·Molecular Biology of the Cell·F C Luca, M Winey
Apr 7, 1998·Molecular and Cellular Biology·S I KomarnitskyC L Denis
Jun 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·A S MahR J Deshaies
Dec 21, 2002·The Journal of Biological Chemistry·Rastislav TamaskovicBrian A Hemmings
Apr 2, 2003·Trends in Cell Biology·Ulrike Gruneberg, Erich A Nigg
Mar 24, 2004·The Journal of Biological Chemistry·Mario R StegertBrian A Hemmings
Apr 7, 2004·The Journal of Biological Chemistry·Eric DevroePamela A Silver
Jun 16, 2004·The Journal of Biological Chemistry·Samuel J BichselBrian A Hemmings
Sep 3, 2004·Current Biology : CB·Andrea KrappViesturs Simanis
May 11, 2005·Cell·William J Bosl, Rong Li
Aug 3, 2005·Cancer Research·John BothosThanos D Halazonetis
Sep 2, 2005·Molecular and Cellular Biology·Alexander HergovichBrian A Hemmings
Apr 12, 2006·Nature Reviews. Molecular Cell Biology·Alexander HergovichBrian A Hemmings
May 6, 2006·Biochemical and Biophysical Research Communications·Alexander HergovichBrian A Hemmings
Feb 24, 2007·Molecular Cell·Alexander HergovichBrian A Hemmings
Feb 24, 2007·Nature Reviews. Cancer·Kieran Harvey, Nicolas Tapon
May 5, 2007·The Journal of Biological Chemistry·Norikazu YabutaHiroshi Nojima
Jul 12, 2007·Nature Reviews. Molecular Cell Biology·Leslie J Saucedo, Bruce A Edgar
Mar 11, 2008·Current Biology : CB·Maria PraskovaJoseph Avruch
Oct 29, 2008·Current Opinion in Cell Biology·Bin ZhaoKun-Liang Guan
Dec 9, 2008·Current Biology : CB·Anton VichalkovskiBrian A Hemmings
Jun 2, 2009·BioFactors·Alexander Hergovich, Brian A Hemmings
Aug 27, 2009·Journal of Cell Science·Ulf R Klein, Erich A Nigg
Dec 23, 2009·Nature Reviews. Molecular Cell Biology·Laura R PearceDario R Alessi

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Citations

Jul 7, 2011·Molecular and Cellular Biology·Yeping ZhouR Jeroen Pasterkamp
Mar 15, 2013·Journal of Visualized Experiments : JoVE·Marta Gomez-MartinezAlexander Hergovich
Aug 18, 2012·Seminars in Cell & Developmental Biology·Joseph AvruchLaura Regué Barrufet
Jun 10, 2015·Scientific Reports·Maomao YanXuebiao Yao
Jan 21, 2012·Biochemical Society Transactions·Alexander Hergovich
May 24, 2016·Genes·Alexander Hergovich
Apr 5, 2017·Molecular & Cellular Proteomics : MCP·Shawn XiongFrank Sicheri
Feb 24, 2016·Cellular Signalling·Lily HoaAlexander Hergovich
Jun 24, 2016·Scientific Reports·Sun-Yong KimToshio Hakoshima
Jun 13, 2019·Cells·Ramazan Gundogdu, Alexander Hergovich
Dec 8, 2015·Journal of Biomolecular Screening·C AgarinisC N Parker
Sep 28, 2017·Nature Communications·Yavuz KulaberogluAlexander Hergovich
Apr 9, 2020·Frontiers in Cell and Developmental Biology·Juan Carlos Duhart, Laurel A Raftery
Mar 30, 2018·Frontiers in Cellular Neuroscience·Adam C O'NeillSilvia Cappello
May 30, 2014·Cancer Research·Fengyuan TangDebora Schmitz-Rohmer
Nov 5, 2020·Cellular and Molecular Life Sciences : CMLS·Alexandre Pj MartinJacques H Camonis
Feb 15, 2012·Journal of Cell Science·Alexandra TavaresHelena Soares
Aug 25, 2021·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Momotaj JahanYutaka Hata
Jan 6, 2016·Genes & Development·Zhipeng MengKun-Liang Guan
May 5, 2011·Cellular Signalling·Alexander Hergovich

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