Differential neural regulation of a neuromuscular junction-associated antigen in muscle fibers and Schwann cells

Journal of Neurobiology
S H AstrowW J Thompson

Abstract

Monoclonal antibodies 3G2 and 4E2 recognize a postsynaptic component of rat neuromuscular junctions. In contrast to many other postsynaptic junctional antigens, expression of this antigen is nerve-dependent: immunoreactivity disappears from junctions following denervation and returns upon reinnervation (Astrow et al., 1992 J. Neurosci. 12:1602-1615). Here we show that the epitope is also expressed by Schwann cells and that this expression is also neurally regulated. Weak mAb 3G2/4E2 immunoreactivity was found in myelinating Schwann cells but was not detected in either nonmyelinating Schwann cells or in terminal Schwann cells at the neuromuscular junction. Following axotomy, immunoreactivity increased in myelinating Schwann cells, and nonmyelinating and terminal Schwann cells became immunopositive. Moreover, the immunoreactivity in terminal Schwann cells revealed their extensive sprouting in response to denervation (Reynolds and Woolf, 1992, J. Neurocytol. 21: 50-66). After nerve regeneration, mAb 3G2/4E2 immunoreactivity in all Schwann cells returned towards normal: it disappeared from terminal Schwann cells, returned to low levels in myelinating Schwann cells, and decreased in nonmyelinating Schwann cells. Immunoblots of axoto...Continue Reading

References

Jan 1, 1991·Glia·K R Jessen, R Mirsky
Jan 1, 1990·Cold Spring Harbor Symposia on Quantitative Biology·U J McMahan
Jul 1, 1991·The Journal of Cell Biology·S C Froehner
Apr 1, 1990·Developmental Biology·K CondonW J Thompson
Jan 1, 1990·Cold Spring Harbor Symposia on Quantitative Biology·J R SanesJ P Merlie
Mar 1, 1988·The American Journal of Physiology·R J Bloch, D W Pumplin
Jan 1, 1987·The Journal of Cell Biology·E A Connor, U J McMahan

❮ Previous
Next ❯

Citations

Jul 1, 1996·Trends in Neurosciences·Y J SonW J Thompson
Sep 23, 2008·Glia·John W Griffin, Wesley J Thompson
Jun 8, 2000·Mechanisms of Development·C Cifuentes-DiazJ P Périn
Jan 1, 1995·Neuron·Y J Son, W J Thompson
Apr 24, 2003·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Daniel S Auld, Richard Robitaille
Jul 1, 2020·Frontiers in Molecular Neuroscience·Young Il Lee, Mendell Rimer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.