Differential Neuronal Plasticity of Dental Pulp Stem Cells From Exfoliated Deciduous and Permanent Teeth Towards Dopaminergic Neurons

Journal of Cellular Physiology
Debanjana MajumdarIndrani Datta

Abstract

Based on early occurrence in chronological age, stem-cells from human exfoliated deciduous teeth (SHED) has been reported to possess better differentiation-potential toward certain cell-lineage in comparison to stem-cells from adult teeth (DPSCs). Whether this same property between them extends for the yield of functional central nervous system neurons is still not evaluated. Hence, we aim to assess the neuronal plasticity of SHED in comparison to DPSCs toward dopaminergic-neurons and further, if the difference is reflected in a differential expression of sonic-hedgehog (SHH)-receptors and basal-expressions of tyrosine-hydroxylase [TH; through cAMP levels]. Human SHED and DPSCs were exposed to midbrain-cues [SHH, fibroblast growth-factor8, and basic fibroblast growth-factor], and their molecular, immunophenotypical, and functional characterization was performed at different time-points of induction. Though SHED and DPSCs spontaneously expressed early-neuronal and neural-crest marker in their naïve state, only SHED expressed a high basal-expression of TH. The upregulation of dopaminergic transcription-factors Nurr1, Engrailed1, and Pitx3 was more pronounced in DPSCs. The yield of TH-expressing cells decreased from 49.8% to 32.16...Continue Reading

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Citations

Jun 18, 2016·Stem Cells International·Vinicius RosaJacques E Nör
Jun 6, 2017·Human Molecular Genetics·A Kaitlyn Victor, Lawrence T Reiter
Sep 22, 2018·Journal of Cellular Physiology·Ghazaleh BaniebrahimiFatemeh Mir
Jan 14, 2020·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Thanasup GonmaneeCharoensri Thonabulsombat
Oct 28, 2019·Current Stem Cell Research & Therapy·Chih-Sheng KoWen-Ta Su
Oct 3, 2020·In Vitro Cellular & Developmental Biology. Animal·Vijay Bhaskar Reddy KonalaRajarshi Pal
May 18, 2018·Frontiers in Bioengineering and Biotechnology·Mona K Marei, Rania M El Backly
Aug 5, 2017·Stem Cells International·Adriana Di BenedettoGiorgio Mori
Feb 1, 2019·International Journal of Molecular Sciences·Christopher SimonSivachandran Parimannan
Jan 8, 2021·Stem Cell Research & Therapy·Zhuangzhuang XiaoHongwu Du
Feb 7, 2018·Archives of Oral Biology·Thanasup GonmaneeHathaitip Sritanaudomchai
Jun 9, 2021·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Pradnya ShahaniIndrani Datta

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