Differential nitric oxide synthase expression during hepatic ischemia-reperfusion

American Journal of Surgery
Ferdinand Serracino-InglottRobert T Mathie

Abstract

In recent years the important role of nitric oxide in hepatic ischemia-reperfusion injury has been increasingly recognised. The prevailing consensus is that reperfusion injury may be partly the result of decreased production of nitric oxide from endothelial nitric oxide synthase and excessive production of nitric oxide from the inducible isoform. We therefore undertook this study to characterize the expression of different nitric oxide synthase isoforms during hepatic reperfusion. Male Wistar rats (n = 6) were subjected to 45 minutes of partial hepatic ischemia (left lateral and median lobes) followed by 6 hours of reperfusion. Control animals (n = 6) were subjected to sham laparotomy. The expression of endothelial and inducible nitric oxide synthase was examined using immunohistochemistry and Western blotting. Liver sections were also stained with nitrotyrosine antibody, a specific marker of protein damage induced by peroxynitrite (a highly reactive free radical formed from nitric oxide). Liver sections from all the control animals showed normal expression of the endothelial isoform and no expression of inducible nitric oxide synthase. Livers from all the animals subjected to hepatic ischemia showed decreased expression of end...Continue Reading

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Citations

Jan 24, 2007·Physiological Reviews·Pál PacherLucas Liaudet
Feb 25, 2010·The Journal of Veterinary Medical Science·Naoto InukaiTatsuya Takizawa
Aug 26, 2015·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Burak Veli UlgerBilsel Bac
Mar 17, 2007·Free Radical Research·Tzong-Jin WuDale A Parks
Oct 5, 2010·Pharmacological Reports : PR·Małgorzata TrochaAdam Szeląg
Jul 8, 2014·Oxidative Medicine and Cellular Longevity·Spyridon SavvanisKassiani Theodoraki
Feb 27, 2007·Free Radical Biology & Medicine·Georgios K GlantzounisAlexander M Seifalian
Aug 27, 2005·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Georgios K GlantzounisAlexander M Seifalian
Feb 10, 2012·Liver International : Official Journal of the International Association for the Study of the Liver·Mahmoud Abu-AmaraBarry Fuller
Apr 21, 2011·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Mahmoud Abu-AmaraBrian Davidson
Jun 25, 2016·Oxidative Medicine and Cellular Longevity·Kassiani TheodorakiVassilios Smyrniotis
May 29, 2015·Acta Cirúrgica Brasileira·Márcio Luís LucasAngelo Alves de Mattos
May 20, 2020·Acta Cirúrgica Brasileira·Tomaz de Jesus Maria Grezzana FilhoCarlos Otavio Corso

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