Differential osteogenicity of multiple donor-derived human mesenchymal stem cells and osteoblasts in monolayer, scaffold-based 3D culture and in vivo

Biomedizinische Technik. Biomedical Engineering
Verena M C QuentJohannes C Reichert

Abstract

We set out to compare the osteogenicity of human mesenchymal stem (hMSCs) and osteoblasts (hOBs). Upon osteogenic induction in monolayer, hMSCs showed superior matrix mineralization expressing characteristic bone-related genes. For scaffold cultures, both cell types presented spindle-shaped, osteoblast-like morphologies forming a dense, interconnected network of high viability. On the scaffolds, hOBs proliferated faster. A general upregulation of parathyroid hormone-related protein (PTHrP), osteoprotegrin (OPG), receptor activator of NF-κB ligand (RANKL), sclerostin (SOST), and dentin matrix protein 1 (DMP1) was observed for both cell types. Simultaneously, PTHrP, RANKL and DMP-1 expression decreased under osteogenic stimulation, while OPG and SOST increased significantly. Following transplantation into NOD/SCID mice, μCT and histology showed increased bone deposition with hOBs. The bone was vascularized, and amounts further increased for both cell types after recombinant human bone morphogenic protein 7 (rhBMP-7) addition also stimulating osteoclastogenesis. Complete bone organogenesis was evidenced by the presence of osteocytes and hematopoietic precursors. Our study results support the asking to develop 3D cellular models cl...Continue Reading

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Citations

Mar 3, 2017·Nature Protocols·Laure C MartineDietmar W Hutmacher
Jul 18, 2017·Journal of Tissue Engineering and Regenerative Medicine·Vmc QuentD Loessner
Oct 6, 2017·Biomedizinische Technik. Biomedical Engineering·Stefan Jockenhoevel
May 27, 2016·Biomedizinische Technik. Biomedical Engineering·Stefan Jockenhoevel

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