Differential Oxygenation in Tumor Microenvironment Modulates Macrophage and Cancer Cell Crosstalk: Novel Experimental Setting and Proof of Concept

Frontiers in Oncology
Noelia CampilloIsaac Almendros

Abstract

Hypoxia is a common characteristic of many solid tumors that has been associated with tumor aggressiveness. Limited diffusion of oxygen generates a gradient of oxygen availability from the blood vessel to the interstitial space and may underlie the recruitment of macrophages fostering cancer progression. However, the available data based on the recruitment of circulating cells to the tumor microenvironment has been so far carried out by conventional co-culture systems which ignore the hypoxic gradient between the vessel to the tumor interstitium. Here, we have designed a novel easy-to-build cell culture device that enables evaluation of cellular cross-talk and cell migration while they are being simultaneously exposed to different oxygenation environments. As a proof-of-concept of the potential role of differential oxygenation among interacting cells we have evaluated the activation and recruitment of macrophages in response to hypoxic melanoma, breast, and kidney cancer cells. We found that hypoxic melanoma and breast cancer cells co-cultured with normoxic macrophages enhanced their directional migration. By contrast, hypoxic kidney cells were not able to increase their recruitment. We also identified well-described hypoxia-in...Continue Reading

References

Mar 4, 2000·The Journal of Pathology·R D LeekA L Harris
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Sep 11, 2002·European Journal of Immunology·Matthew J GrimshawFrances R Balkwill
Jan 1, 2003·The American Journal of Pathology·Susanne MatschuratMatthias Clauss
Sep 18, 2003·Nature Reviews. Cancer·Gregg L Semenza
Dec 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·J Clifford MurrayR Adrian Robins
Jul 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Thorsten HagemannFrances R Balkwill
Feb 20, 2010·Nature Reviews. Cancer·Dingzhi Wang, Raymond N Dubois
May 25, 2011·Nature Reviews. Cancer·William R Wilson, Michael P Hay
Jun 10, 2011·Nature·Bin-Zhi QianJeffrey W Pollard
Oct 8, 2011·PloS One·Alexander B HermanGeoffrey B West
Mar 20, 2012·PloS One·Lev BeckerJay W Heinecke
Nov 2, 2012·Immunology·Xiaoqiang TangHengyi Xiao
Jan 5, 2013·Biochimica Et Biophysica Acta·Heon-Woo LeeYoung-Guen Kwon
Jul 3, 2013·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Yuan ZhangAnita Sjölander
Nov 10, 2013·Nature Medicine·Daniela F Quail, Johanna A Joyce
Jan 30, 2014·American Journal of Respiratory and Critical Care Medicine·Isaac AlmendrosDavid Gozal
Mar 15, 2014·JAMA Ophthalmology·Inge H G BronkhorstMartine J Jager
Apr 20, 2014·Oncoimmunology·Eva Van OvermeireJo A Van Ginderachter
May 7, 2014·Proceedings of the National Academy of Sciences of the United States of America·Pallavi ChaturvediGregg L Semenza
May 16, 2014·Nature Reviews. Cancer·Daniele M GilkesDenis Wirtz
Aug 15, 2014·Neoplasia : an International Journal for Oncology Research·Einar K RofstadBerit S Mathiesen
Aug 16, 2014·Cancers·Theerawut ChanmeeNaoki Itano
Oct 6, 2014·Trends in Biotechnology·Matthew B ByrnePaul J A Kenis
Jan 15, 2015·Biomicrofluidics·Miguel A AcostaMichael P Gamcsik
Mar 22, 2016·NPJ Breast Cancer·Carly Bess WilliamsAdam C Soloff
Jul 15, 2016·Cancer Cell·Claire E LewisJeffrey W Pollard
Aug 4, 2016·Proceedings of the National Academy of Sciences of the United States of America·Daniel M LewisSharon Gerecht
Nov 1, 2016·Scientific Reports·Jose M AyusoIgnacio Ochoa
Nov 1, 2016·Acta Biochimica Et Biophysica Sinica·Bingyan LiuAiwu Mao
Jan 4, 2017·Scientific Reports·Edoardo MilottiRoberto Chignola

❮ Previous
Next ❯

Citations

Feb 6, 2020·Journal of Clinical Sleep Medicine : JCSM : Official Publication of the American Academy of Sleep Medicine·Arthur SillahAmanda I Phipps
Oct 6, 2020·Frontiers in Bioengineering and Biotechnology·Somshuvra BhattacharyaPilar de la Puente
Jul 22, 2019·Cancers·Niels J BrouwerMartine J Jager
Sep 15, 2020·Current Opinion in Pulmonary Medicine·Oreste Marrone, Maria R Bonsignore
Feb 3, 2021·Cancers·Tord HomplandHeidi Lyng
Dec 19, 2020·International Journal of Molecular Sciences·Linhan ZhuangMartina Niebler
Jun 3, 2021·International Journal of Molecular Sciences·David Sanz-RubioDavid Gozal
Jul 3, 2021·Journal of Cellular and Molecular Medicine·Min Hee KimHeiko Konig
Aug 28, 2021·Antioxidants·Jorge OteroIsaac Almendros

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence-activated cell sorting
chip
fluorescence activated cell sorting
FACS
PCR

Software Mentioned

FlowJo
SigmaPlot
Autodesk 123D Design
ImageJ
FACS Diva

Related Concepts

Related Feeds

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.