Differential Phosphoproteomic Analysis of Recombinant Chinese Hamster Ovary Cells Following Temperature Shift

Journal of Proteome Research
Michael HenryPaula Meleady

Abstract

Phosphorylation is one of the most important post-translational modifications, playing a crucial role in regulating many cellular processes, including transcription, cytoskeletal rearrangement, cell proliferation, differentiation, apoptosis, and signal transduction. However, to date, little work has been carried out on the phosphoproteome in CHO cells. In this study we have carried out a large scale differential phosphoproteomic analysis of recombinant CHO cells following a reduction of culture temperature (temperature shift). The reduction of culture temperature during the exponential phase of growth is commonly employed by the biopharmaceutical industry to increase product yield; however, the molecular mechanisms of temperature shift in CHO cells remain poorly understood. We have identified 700 differentially expressed phosphopeptides using quantitative label-free LC-MS/MS phosphoproteomic analysis in conjunction with IMAC and TiO2 phosphopeptide enrichment strategies, following a reduction in temperature from 37 to 31 °C. Functional assessment of the phosphoproteomic data using gene ontology analysis showed a significant enrichment of biological processes related to growth (e.g., cell cycle, cell division), ribosomal biogene...Continue Reading

References

Jun 22, 1999·Oncogene·F De GraeveB Chatton
Jul 9, 1999·Biotechnology and Bioengineering·H KaufmannJ E Bailey
Apr 25, 2000·The Journal of Biological Chemistry·S Y FuchsZ Ronai
Aug 21, 2001·Genes & Development·S PyronnetN Sonenberg
Nov 24, 2001·The Journal of Biological Chemistry·Constantinos G BroustasUsha Kasid
Sep 21, 2004·The Journal of Biological Chemistry·Susanne SteinPeng Liang
Feb 3, 2005·Biotechnology and Bioengineering·Michèle F UnderhillLouise H Naylor
Dec 23, 2006·Annals of the New York Academy of Sciences·Atsushi EnomotoMasahide Takahashi
Oct 24, 2007·Nature Methods·Lukas KällMichael J MacCoss
Oct 7, 2008·Annual Review of Pharmacology and Toxicology·Boris MacekJesper V Olsen
May 26, 2009·Biotechnology Advances·David L HackerFlorian M Wurm
Aug 12, 2009·Analytical Chemistry·Tyler CarlageWilliam S Hancock
May 13, 2010·Nature Reviews. Molecular Cell Biology·Chunaram Choudhary, Matthias Mann
Jun 10, 2011·The Journal of Biological Chemistry·Sushma Iyengar, Peggy J Farnham
Jun 29, 2011·Biochemical and Biophysical Research Communications·Catherine McCaigJames T Murray
Nov 15, 2011·Journal of Proteome Research·Thomas TausKarl Mechtler
Jan 6, 2012·The Biochemical Journal·Sébastien Pyr Dit RuysChristopher G Proud
Mar 6, 2012·Biotechnology and Bioengineering·Paula MeleadyNicole Borth
Apr 14, 2012·Chemical Society Reviews·Thomas E AngelRichard D Smith
Sep 19, 2012·Journal of Proteome Research·Ryohei NarumiTakeshi Tomonaga
Oct 13, 2012·The Journal of Biological Chemistry·Lu ChenW Douglas Cress
Jan 17, 2013·Biotechnology and Bioengineering·Payel DattaSusan T Sharfstein
Mar 1, 2013·Molecular Biology of the Cell·Noah DephoureDouglas R Kellogg

❮ Previous
Next ❯

Citations

Aug 20, 2019·Applied Microbiology and Biotechnology·Louise SchelletterRaimund Hoffrogge
Jun 25, 2020·Current Research in Biotechnology·Hussain DahodwalaSusan T Sharfstein

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis