Differential pressure resistance in the activity of RNA polymerase isolated from Shewanella violacea and Escherichia coli

Extremophiles : Life Under Extreme Conditions
Hiroaki KawanoFumiyoshi Abe

Abstract

RNA polymerase was purified from the piezophile Shewanella violacea DSS12, and the transcriptional activity after pressure treatment was compared with that of the mesophile Escherichia coli. Application of pressure at 100 MPa for 30 min reduced the E. coli RNA polymerase activity to 60% of the activity at atmospheric pressure, whereas the S. violacea RNA polymerase maintained full activity, indicating that the S. violacea RNA polymerase is more stable than its E. coli counterpart. This result was supported by the analysis of the strength of subunit interactions of the enzyme from both species, using a high-pressure electrophoresis apparatus, which showed that a pressure of 140 MPa caused dissociation of E. coli RNA polymerase but not that of S. violacea RNA polymerase. On the other hand, the core enzyme of S. violacea RNA polymerase, which lacked the sigma70 factor, was dissociated at 140 MPa. These results suggest that the sigma70 factor is required for stabilization of S. violacea RNA polymerase under high-pressure conditions. In this paper, we provide in vitro evidence for piezoadaptation at the transcriptional level, using purified RNA polymerase from cells of S. violacea and E. coli.

References

Jan 1, 1979·Annual Review of Genetics·T Yura, A Ishihama
Nov 1, 1992·Bioscience, Biotechnology, and Biochemistry·M Fujita, A Amemura
Mar 1, 1988·Journal of Biochemical and Biophysical Methods·K P Kumar, D Chatterji
Oct 20, 1995·Journal of Molecular Biology·L Erijman, R M Clegg
Jan 1, 1993·Annual Review of Physical Chemistry·J L Silva, G Weber
Jan 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·D P GreinerC F Meares
Oct 23, 1998·Extremophiles : Life Under Extreme Conditions·K NakasoneK Horikoshi
Nov 5, 1999·Trends in Microbiology·F AbeK Horikoshi
May 2, 2002·Biochimica Et Biophysica Acta·D H Bartlett
Oct 9, 2002·Nature Biotechnology·John F HeidelbergClaire M Fraser

❮ Previous
Next ❯

Citations

Nov 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jack MerrinAlbert Libchaber
Dec 31, 2009·Journal of Biochemistry·Chiho MurakamiChiaki Kato
Jun 3, 2008·Nucleic Acids Research·David J WiltonMike P Williamson
Aug 27, 2005·The Journal of Chemical Physics·P M W GillM Head-Gordon
Sep 8, 2011·Bioscience, Biotechnology, and Biochemistry·Misa MasanariYoshihiro Sambongi
Apr 5, 2014·Extremophiles : Life Under Extreme Conditions·Misa MasanariYoshihiro Sambongi
Jul 26, 2005·Bioscience, Biotechnology, and Biochemistry·Hiroaki KawanoKoki Horikoshi
Mar 18, 2010·Annals of the New York Academy of Sciences·Douglas H Bartlett
Jul 9, 2013·Biotechnology Advances·Maria J MotaJorge A Saraiva
Jun 24, 2015·Extremophiles : Life Under Extreme Conditions·Mohamed JebbarPhilippe Oger
Jun 6, 2009·Trends in Biotechnology·Abram AertsenChris W Michiels
Sep 21, 2016·Bioscience, Biotechnology, and Biochemistry·Misa MasanariYoshihiro Sambongi
Mar 16, 2018·Bioscience, Biotechnology, and Biochemistry·Sotaro FujiiYoshihiro Sambongi
Mar 5, 2010·Prikladnaia biokhimiia i mikrobiologiia·E V MorozkinaO V Koroleva
Aug 14, 2019·International Journal of Biological Macromolecules·Yanan Li, Haibin Zhang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.