Differential production of interleukin-12 mRNA by murine macrophages in response to viable or killed Salmonella spp.

Infection and Immunity
C ChongJ D Clements

Abstract

The use of attenuated Salmonella spp. as live oral vaccine carriers fo r foreign antigens has been extensively studied. We have shown that appropriately prepared nonviable organisms are as effective as viable organisms in eliciting humoral immune responses against a foreign antigen delivered by these vectors. It is not clear how strain viability affects the development of a cell-mediated immune response. In the present study, we demonstrate that BALB/c mice orally immunized with viable attenuated Salmonella spp. were protected against subsequent challenge while animals immunized with killed organisms were not. Protection was correlated with increased production of interleukin-12 (IL-12) p40 mRNA in the Peyer's patches within hours of oral administration. Peritoneal macrophages from lipopolysaccharide (LPS)-responsive and LPS-unresponsive mice were also examined for production of IL-12 p40 mRNA following exposure to the viable or killed attenuated Salmonella carrier. There was dramatic upregulation of IL-12 p40 mRNA following exposure of macrophages to either viable or killed organisms. By 4 h postexposure, viable organisms had induced a 27-fold increase in IL-12 p40 mRNA levels while killed organisms had induced a 9-fold increa...Continue Reading

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Citations

Jul 9, 2009·Bioscience, Biotechnology, and Biochemistry·Shintaro IchikawaYutaka Konishi
Jun 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·I Marriott, K L Bost
Sep 7, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·A ElhofyK L Bost
Sep 24, 2005·Infection and Immunity·R William DepaoloWilliam J Karpus
Sep 9, 2011·Clinical and Vaccine Immunology : CVI·Laura StoekerGregg A Dean
Sep 24, 1998·Infection and Immunity·R A Van AndelL K Riley
Dec 4, 2003·FEMS Immunology and Medical Microbiology·Alfonso Ruiz-BravoMaria Jimenez-Valera
Dec 28, 1999·Microbes and Infection·A C Lalmanach, F Lantier
Oct 23, 1997·Clinical Microbiology Reviews·L RomaniF Bistoni
Sep 14, 2007·BMC Microbiology·Wendy RodenburgIngeborg M J Bovee-Oudenhoven

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