Differential production of superoxide by neuronal mitochondria

BMC Neuroscience
Mark J HoeggerLeonard A Levin

Abstract

Mitochondrial DNA (mtDNA) mutations, which are present in all mitochondria-containing cells, paradoxically cause tissue-specific disease. For example, Leber's hereditary optic neuropathy (LHON) results from one of three point mutations mtDNA coding for complex I components, but is only manifested in retinal ganglion cells (RGCs), a central neuron contained within the retina. Given that RGCs use superoxide for intracellular signaling after axotomy, and that LHON mutations increase superoxide levels in non-RGC transmitochondrial cybrids, we hypothesized that RGCs regulate superoxide levels differently than other neuronal cells. To study this, we compared superoxide production and mitochondrial electron transport chain (METC) components in isolated RGC mitochondria to mitochondria isolated from cerebral cortex and neuroblastoma SK-N-AS cells. In the presence of the complex I substrate glutamate/malate or the complex II substrate succinate, the rate of superoxide production in RGC-5 cells was significantly lower than cerebral or neuroblastoma cells. Cerebral but not RGC-5 or neuroblastoma cells increased superoxide production in response to the complex I inhibitor rotenone, while neuroblastoma but not cerebral or RGC-5 cells dramat...Continue Reading

References

May 15, 1976·The Biochemical Journal·A BoverisA O Stoppani
Nov 1, 1980·The Biochemical Journal·J F Turrens, A Boveris
May 25, 1995·Biochemical and Biophysical Research Communications·L VerganiE Lugaresi
Feb 21, 2002·Human Molecular Genetics·Alice WongGino A Cortopassi
Feb 26, 2003·Investigative Ophthalmology & Visual Science·Xiaoping QiJohn Guy
Oct 29, 2003·Antioxidants & Redox Signaling·Steve M NguyenLeonard A Levin
Nov 20, 2003·The Journal of Biological Chemistry·Alexei P KudinWolfram S Kunz
Feb 25, 2005·Brain : a Journal of Neurology·Steven R DanielsonGino A Cortopassi
Sep 28, 2005·Investigative Ophthalmology & Visual Science·Kyle I SwansonLeonard A Levin
Oct 26, 2005·The Journal of Biological Chemistry·Alexander PanovJ Timothy Greenamyre
Dec 31, 2005·Investigative Ophthalmology & Visual Science·Laura J FrassettoLeonard A Levin
Mar 28, 2006·Investigative Ophthalmology & Visual Science·Christopher J LievenLeonard A Levin
May 10, 2006·Proceedings of the National Academy of Sciences of the United States of America·Lothar Kussmaul, Judy Hirst

❮ Previous
Next ❯

Citations

May 25, 2010·Brain : a Journal of Neurology·Akiyasu KanamoriLeonard A Levin
Aug 5, 2009·The Journal of Bone and Joint Surgery. American Volume·James A MartinTodd O McKinley
Feb 20, 2009·Journal of Glaucoma·George Y X KongJonathan G Crowston
May 18, 2013·Disability and Rehabilitation·Stephen A BuetowSuzanne Purdy
May 9, 2015·Journal of Clinical and Experimental Neuropsychology·Juliette PalissonPauline Narme
Apr 6, 2013·Fertility and Sterility·Yao-Lung ChangTzu-Hao Wang
Jul 31, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Daraporn Chua-OnSiriporn Proungvitaya
Jun 7, 2008·The Journal of International Medical Research·J Y WangY Tong
May 2, 2019·Scientific Reports·Razek Georges CoussaLeonard A Levin

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

iCycler
NIH ImageJ

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.