Differential profiling of lacrimal cytokines in patients suffering from thyroid-associated orbitopathy

Scientific Reports
Edina KishaziMehrad Hamedani

Abstract

The aim was to investigate the levels of cytokines and soluble IL-6R in the tears of patients with thyroid-associated orbitopathy (TAO) disease. Schirmer's test was adopted to collect tears from TAO patients (N = 20, 17 women, mean age (±SD): 46.0 years (±13.4)) and healthy subjects (N = 18, 10 women, 45.4 years (±18.7)). Lacrimal cytokines and soluble IL-6R (sIL-6R) were measured using a 10-plex panel (Meso Scale Discovery Company) and Invitrogen Human sIL-6R Elisa kit, respectively. Tear levels of IL-10, IL-12p70, IL-13, IL-6 and TNF-α appeared significantly higher in TAO patients than in healthy subjects. Interestingly, IL-10, IL-12p70 and IL-8 levels increased in tears whatever the form of TAO whereas IL-13, IL-6 and TNF-α levels were significantly elevated in inflammatory TAO patients, meaning with a clinical score activity (CAS) ≥ 3, compared to controls. Furthermore, only 3 cytokines were strongly positively correlated with CAS (IL-13 Spearman coeff. r: 0.703, p = 0.0005; IL-6 r: 0.553, p = 0.011; IL-8 r: 0.618, p = 0.004, respectively). Finally, tobacco use disturbed the levels of several cytokines, especially in patient suffering of TAO. The differential profile of lacrimal cytokines could be useful for the diagnosis o...Continue Reading

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Citations

Jan 24, 2019·Pharmaceutical Research·Mitalee TamhaneVeena Viswanath
Jul 18, 2019·Current Opinion in Ophthalmology·Jonathan C P Roos, Rachna Murthy
Apr 22, 2020·Expert Review of Ophthalmology·Jackeline Rodriguez-SmithSheila Angeles-Han
Jan 28, 2021·International Journal of Molecular Sciences·Jeong-Sun HanDong-Jun Lim
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Nov 10, 2021·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·M Hossein NowroozzadehReza Razeghinejad
Jan 30, 2021·European Thyroid Journal·Alan Chun Hong Lee, George J Kahaly

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Methods Mentioned

BETA
ELISA

Software Mentioned

GraphPad Prism
GraphPad

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