Differential proliferative response of fetal and adult human skin fibroblasts to transforming growth factor-beta

Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society
Harris PratsinisDimitris Kletsas

Abstract

Since pronounced differences exist between the fetal and adult repair processes, we studied the proliferative response of skin fibroblasts from these two stages to transforming growth factor-beta (TGF-beta), a cytokine with a broad range of activities in tissue repair. Here, we present evidence that TGF-beta inhibits fetal human skin fibroblasts, while it is stimulatory for adult ones. This proliferative effect of TGF-beta was found to be concentration- dependent, but isoform-independent. Furthermore, even a transient exposure of the cells to this growth factor was sufficient to exert its stimulatory or inhibitory action. Accordingly, we have studied the immediate responses provoked by TGF-beta in major signaling pathways, and we have found that it induces a rapid activation of the SMAD pathway, i.e., phosphorylation and nuclear translocation of SMAD2, followed by dephosphorylation, most probably due to degradation by the proteasome. However, similar intensity and kinetics of this activation have been observed in both fetal and adult fibroblasts. On the other hand, curcumin, a natural product with wound healing properties that inhibits several intracellular signaling pathways, was found to completely abrogate the inhibitory eff...Continue Reading

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Citations

Sep 4, 2007·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Harris Pratsinis, Dimitris Kletsas
Jul 15, 1998·Mechanisms of Ageing and Development·D KletsasE Ottaviani
Jun 15, 2006·Plastic and Reconstructive Surgery·Amy S ColwellH Peter Lorenz
Jan 16, 2014·Disease Markers·Fernanda Rodrigues HelmoRosana Rosa Miranda Corrêa
May 1, 2007·Regenerative Medicine·Dominic FrimbergerBradley P Kropp
Jun 8, 2012·ISRN Dermatology·K J Rolfe, A O Grobbelaar
Apr 14, 2015·Periodontology 2000·Matthias ChiquetDimitris Kletsas
Aug 10, 2007·The Journal of Investigative Dermatology·Kerstin J RolfeClaire Linge
Sep 20, 2015·Pathology, Research and Practice·Fernanda Rodrigues HelmoRosana Rosa Miranda Corrêa
Aug 31, 2006·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Sandeep KathjuGarth D Ehrlich
Sep 16, 2010·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Benjamin J HerdrichKenneth W Liechty
Oct 16, 2010·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Stefania ChrissouliDimitris Kletsas
Mar 24, 2010·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Eleni Mavrogonatou, Dimitris Kletsas
Oct 19, 2013·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Mariëlle WalravenMagda M W Ulrich
Mar 22, 2015·Journal of Dermatological Science·M WalravenM M W Ulrich
Jul 20, 2012·Biology of Reproduction·Dulce Maroni, John S Davis
Mar 3, 2018·Laboratory Investigation; a Journal of Technical Methods and Pathology·Caroline L HallKai P Leung
Jan 1, 2014·Comprehensive Reviews in Food Science and Food Safety·Seyed Fazel NabaviSeyed Mohammad Nabavi

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