Differential properties of GTP- and Ca(2+)-stimulated exocytosis from large dense core vesicles

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Li BaiTao Xu

Abstract

Many cells utilize a GTP-dependent pathway to trigger exocytosis in addition to Ca(2+)-triggered exocytosis. However, little is known about the mechanism by which GTP triggers exocytosis independent of Ca(2+). We used dual-color evanescent field microscopy to compare the motion and fusion of large dense core vesicles stimulated by either mastoparan (Mas) in Ca(2+)-free conditions or high K(+) in the presence of Ca(2+). We demonstrate that Mas is hardly effective in triggering the fusion of the predocked vesicles but predominantly mobilizes cytosolic vesicles. In contrast, Ca(2+)-dependent exocytosis is largely due to predocked vesicles. Fusion kinetics analysis and carbon-fiber amperometry reveal that Mas induces a brief 'kiss-and-run' fusion and releases only a small amount of the cargo, whereas Ca(2+) stimulates a more persistent opening of the fusion pore and larger release of the contents. Furthermore, we show that Mas-released vesicles require a much shorter time to reach fusion competence once they approach the plasma membrane. Our data suggest the involvement of different mechanisms not only in triggering and fusion but also in the docking and priming process for Ca(2+)- and GTP-dependent exocytosis.

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Citations

Jul 14, 2009·Pflügers Archiv : European journal of physiology·Renhao XuePeng Chen
Dec 18, 2009·Science in China. Series C, Life Sciences·JiangLi LiTao Xu
Jun 16, 2009·Neuroscience Research·Volkmar Lessmann, Tanja Brigadski
May 13, 2008·Biochemical and Biophysical Research Communications·Zhi-Tao HuZheng-Xing Wu
Aug 10, 2007·Biochemical and Biophysical Research Communications·Dan ZhuTao Xu
Jan 23, 2008·Traffic·Alejandra TomasPhilippe A Halban
Oct 19, 2006·Biochemical and Biophysical Research Communications·Pingyong XuTao Xu
Jun 20, 2015·PloS One·Nikhil R GandasiSebastian Barg
Oct 18, 2012·Physiological Reviews·Haruo KasaiHiroshi Tokumaru
Sep 7, 2013·Science China. Life Sciences·Lina PengWenqiang Yu
Oct 4, 2015·The Journal of Biological Chemistry·Masaki YamagaThomas F J Martin

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