Differential protein expression following low temperature culture of suspension CHO-K1 cells.

BMC Biotechnology
Niraj KumarMartin Clynes

Abstract

To ensure maximal productivity of recombinant proteins (rP) during production culture it is typical to encourage an initial phase of rapid cell proliferation to achieve high biomass followed by a stationary phase where cellular energies are directed towards production of rP. During many such biphasic cultures, the initial phase of rapid cell growth at 37 degrees C is followed by a growth arrest phase induced through reduction of the culture temperature. Low temperature induced growth arrest is associated with many positive phenotypes including increased productivity, sustained viability and an extended production phase, although the mechanisms regulating these phenotypes during mild hypothermia are poorly understood. In this study differential protein expression in suspension CHO-K1 cells was investigated following a reduction of the culture temperature from 37 degrees C to 31 degrees C in comparison to standard batch culture maintained at 37 degrees C using 2D-DIGE (Fluorescence 2-D Difference Gel Electrophoresis) and mass spectrometry (MS). There is only limited proteomic analysis of suspension-grown CHO cells describing a direct comparison of temperature shifted versus non-temperature shifted cultures using 2D-DIGE. This inv...Continue Reading

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Citations

Dec 14, 2011·Applied Microbiology and Biotechnology·Jee Yon KimGyun Min Lee
Jul 20, 2011·Biotechnology Letters·Christopher J HewittColin R Thomas
Dec 24, 2011·BMC Genomics·Michael A J ZiegerMu Wang
Mar 24, 2010·BMC Biotechnology·Darrin KuystermansMohamed Al-Rubeai
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Sep 1, 2020·Biotechnology and Bioengineering·Camila A OrellanaEsteban Marcellin
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Feb 25, 2020·Biotechnology and Applied Biochemistry·Reyhane KeshavarzAli Asghar Deldar
Jan 23, 2021·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Pablo Vicente Torres-OrtegaMaría J Blanco-Prieto

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Methods Mentioned

BETA
electrophoresis
protein assay

Software Mentioned

ImageQuant
Ettan MALDI
Profound
Ettan MALDI - ToF Pro evaluation
Decyder
ImageMaster Platinum

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