Differential protein expression in Colletotrichum acutatum: changes associated with reactive oxygen species and nitrogen starvation implicated in pathogenicity on strawberry.

Molecular Plant Pathology
Sigal Horowitz BrownStanley Freeman

Abstract

The cellular outcome of changes in nitrogen availability in the context of development and early stages of pathogenicity was studied by quantitative analysis of two-dimensional gel electrophoresis of Colletotrichum acutatum infecting strawberry. Significant alterations occurred in the abundance of proteins synthesized during appressorium formation under nitrogen-limiting conditions compared with a complete nutrient supply. Proteins that were up- or down-regulated were involved in energy metabolism, nitrogen and amino acid metabolism, protein synthesis and degradation, response to stress and reactive oxygen scavenging. Members belonging to the reactive oxygen species (ROS) scavenger machinery, superoxide dismutase and glutathione peroxidase, were up-regulated at the appressorium formation stage, as well as under nitrogen-limiting conditions relative to growth with a complete nutrient supply, whereas abundance of bifunctional catalase was up-regulated predominantly at the appressorium formation stage. Fungal ROS were detected within germinating conidia during host pre-penetration, penetration and colonization stages, accompanied by plant ROS, which were abundant in the apoplastic space. Application of exogenous antioxidants quenc...Continue Reading

References

Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·D B JenningsJ D Williamson
Oct 26, 1999·The Journal of Peptide Research : Official Journal of the American Peptide Society·P Dolashka-AngelovaW Voelter
Feb 15, 2002·The Plant Journal : for Cell and Molecular Biology·Denny G MellershMichele C Heath
Oct 14, 2003·FEMS Microbiology Letters·Young-sil HaMartin B Dickman
Jun 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Ryoung Shin, Daniel P Schachtman
Aug 13, 2004·Molecular Microbiology·Peter S SolomonRichard P Oliver
Sep 21, 2004·Annual Review of Plant Biology·Klaus Apel, Heribert Hirt
Feb 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Changbin Chen, Martin B Dickman
Feb 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·Rusty Rodriguez, Regina Redman
Mar 2, 2005·Trends in Microbiology·Jesús AguirreWilhelm Hansberg
Mar 25, 2005·Biochemical Society Transactions·Z-Y WangN J Talbot
Mar 18, 2006·Proteomics·Francisco Javier Fernández-AceroJesús Jorrín
May 30, 2006·Fungal Genetics and Biology : FG & B·N M DonofrioR A Dean
Sep 8, 2006·Applied and Environmental Microbiology·Makoto AsakuraYoshitaka Takano
Nov 7, 2006·FEMS Microbiology Letters·Hege H Divon, Robert Fluhr
Jun 30, 2007·Proceedings of the National Academy of Sciences of the United States of America·Martin J EganNicholas J Talbot
Oct 24, 2008·Phytopathology·Kenneth J CurryBarbara J Smith

❮ Previous
Next ❯

Citations

Feb 2, 2013·Archives of Microbiology·Mohamed Rabie El-AkhalFrancisco Javier Fernández-Acero
Nov 20, 2012·Journal of Proteomics·Sang Gon KimKyu Young Kang
Jun 13, 2013·Journal of Proteomics·Kristin KrollOlaf Kniemeyer
Nov 4, 2010·Molecular Plant-microbe Interactions : MPMI·Shigeru TanabeEiichi Minami
Dec 14, 2019·World Journal of Microbiology & Biotechnology·Lihua TangTom Hsiang
Nov 4, 2020·Archives of Insect Biochemistry and Physiology·Dana MentItamar Glazer
May 14, 2011·Journal of Proteome Research·Yiming WangKyu Young Kang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.