Differential protein expression in white adipose tissue from obesity-prone and obesity-resistant mice in response to high fat diet and anti-obesity herbal medicines

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Sang Woo KimJong Won Yun

Abstract

One of the most interesting issues in obesity research is why certain humans are obesity-prone (OP) while others are obesity-resistant (OR) upon exposure to a high-calorie diet. However, the pathways responsible for these phenotypic differences are still largely unknown. In order to discover marker molecules determining susceptibility and/or resistance to obesity in response to high fat diet (HFD) or anti-obesity herbal medicine (TH), we conducted comparative proteomic analysis of white adipose tissue (WAT) from OP, OR, as well as TH-treated mice. OP mice fed HFD gained approximately 33% more body weight than OR mice, and TH significantly reduced body weight gain in HFD-fed mice by 30%. These mice were further subjected to proteomic analysis using two-dimensional electrophoresis (2-DE) combined with matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS). Proteomic data revealed 59 spots that were differentially regulated from a total of 1,045 matched spots, and 57 spots of these were identified as altered WAT proteins between OP and OR mice by peptide mass finger printing. Interestingly, 45 proteins were similarly regulated in OR mice in response to TH treatment. Of these, 10 proteins have a...Continue Reading

Citations

Jun 30, 2015·International Journal of Molecular Sciences·Hilal Ahmad Parray, Jong Won Yun
Feb 26, 2016·Molecular and Cellular Biochemistry·Sang Woo KimJong Won Yun
Sep 20, 2018·Evidence-based Complementary and Alternative Medicine : ECAM·Sae-Rom YooHyekyung Ha

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