Differential protein profiling as a potential multi-marker approach for TSE diagnosis.

BMC Infectious Diseases
Janice B BarrRona Barron

Abstract

Transmissible spongiform encephalopathy describes a family of diseases affecting both man and animals. Current tests for the diagnosis of these diseases are based on the detection of an abnormal misfolded form of the host protein PrP which is found within the central nervous and lymphoreticular systems of affected animals. Recently, concern that this marker may not be as reliable as previously thought, coupled with an urgentneed for a pre-clinical live animal test, has led to the search for alternative assays for the detection of TSE disease. This "proof of concept" study, examines the use of differential protein expression profiling using surface enhanced laser desorption and ionisationtime of flight mass spectrometry (SELDI-TOF) for the diagnosis of TSE disease. Spectral output from all proteins selectively captured from individual murine brain homogenate samples, are compared as "profiles" in groups of infected and non-infected animals. Differential protein expression between groups is thus highlighted and statistically significant protein "peaks" used to construct a panel of disease specific markers.Studies at both terminal stages of disease and throughout the time course of disease have shown a disease specific protein pro...Continue Reading

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Citations

Nov 4, 2010·BMC Veterinary Research·Isabelle Batxelli-MolinaChantal Mourton-Gilles
Mar 24, 2012·BMC Veterinary Research·Miriam G J KoeneMari A Smits
Aug 12, 2010·Archives of Physiology and Biochemistry·Janice BarrRona M Barron
Apr 13, 2011·Comparative Immunology, Microbiology and Infectious Diseases·L ZhongR J Whittington
Dec 22, 2012·Expert Review of Proteomics·Edmondo CampisiMaurizio Pocchiari

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Methods Mentioned

BETA
protease digest
electrophoresis
biopsy

Software Mentioned

ProteinChip ®
Ciphergen Express
ProteinChip
Mascot Sciences
R
ProteinChip ® Reader
Biomarker Wizard

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