Differential Protein Profiling of Cerebrospinal Fluid in Piglets with Severe Meningoencephalitis Caused by Streptococcus suis Type 2 Compared to Controls

Frontiers in Cellular and Infection Microbiology
Hongtao LiuLiancheng Lei

Abstract

Streptococcus suis serotype 2 (SS2) is a zoonotic pathogen that can cause meningitis both in pigs and in human beings. However, the pathogenesis of central nervous system (CNS) infection caused by SS2 have not yet been elucidated. To find the key molecules in cerebrospinal fluid (CSF) needed for the pathogenesis, a SS2 meningoencephalitic pig model and a SS2 non-meningoencephalitic pig model were established in this study. CSF was collected from infected piglets, and protein profiling was performed with label-free proteomics technology. A total of 813 differential proteins, including 52 up-regulated proteins and 761 down-regulated proteins, were found in the CSF of meningoencephalitic pigs compared with both non-meningoencephalitic pigs and healthy pigs. These 813 differential proteins were clustered into three main categories, namely, cellular component, biological process, and molecular function by gene ontology (GO) analysis. The most enriched subclasses of differential proteins in each category were exosome (44.3%), energy pathway (25.0%) and catalytic activity (11.3%), respectively. The most enriched subclasses of upregulated proteins were extracellular (62.1%), protein metabolism (34.5%) and cysteine-type peptidase activi...Continue Reading

References

Mar 1, 1989·Somatic Cell and Molecular Genetics·M RocchiV Colantuoni
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·G R MooreN K Rogers
Nov 15, 1996·Genes & Development·K WangS J Korsmeyer
Jul 20, 2000·Journal of Neuropathology and Experimental Neurology·N Strazielle, J F Ghersi-Egea
Jan 12, 2002·Biochemical and Biophysical Research Communications·Wolfgang H Goldmann, Donald E Ingber
Jan 24, 2002·The Journal of Biological Chemistry·Sunil K Sukumaran, Nemani V Prasadarao
Jan 30, 2002·Journal of Comparative Pathology·L W MadsenH E Jensen
Sep 17, 2002·Briefings in Bioinformatics·Claire O'DonovanRolf Apweiler
Jan 10, 2003·Nucleic Acids Research·Cathy H WuWinona C Barker
Jan 10, 2003·Nucleic Acids Research·Brigitte BoeckmannMichel Schneider
Aug 2, 2007·Analytical and Bioanalytical Chemistry·Marcus BantscheffBernhard Kuster
Mar 1, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Nguyen Thi Hoang MaiConstance Schultsz
Jun 25, 2010·Molecular Biology of the Cell·Douwe M Veltman, Robert H Insall
Oct 26, 2011·Biochimica Et Biophysica Acta·Vito TurkDušan Turk
Oct 2, 2013·The Journal of Biological Chemistry·Minseon ParkMichal Toborek
Apr 9, 2015·Frontiers in Neuroscience·Christian B BrøchnerKjeld Møllgård
Oct 28, 2015·PLoS Neglected Tropical Diseases·Anusha van SamkarDiederik van de Beek
Dec 20, 2015·Journal of Neuropathology and Experimental Neurology·Marvin M van LuijnRogier Q Hintzen
Jul 28, 2016·Cellular Microbiology·Nicole de BuhrMaren von Köckritz-Blickwede
Jan 17, 2017·Nature Reviews. Microbiology·Mathieu CoureuilXavier Nassif
Jul 6, 2017·Proteomics. Clinical Applications·Nikita GahoiSanjeeva Srivastava
Sep 25, 2017·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Jean DejaceEmily Wood

❮ Previous
Next ❯

Methods Mentioned

BETA
enzyme-linked immunosorbent assay
protein folding
GTPase
ELISA

Software Mentioned

Maxquant

Related Concepts

Related Feeds

Alzheimer's Disease: Endosomes

Dysfunctional endosomal trafficking may be associated with Alzheimer’s disease (AD) pathology. Targeting the endosome may advance treatment options for AD. Here is the latest research on endosomes and AD.

CSF & Lymphatic System

This feed focuses on Cerebral Spinal Fluid (CSF) and the lymphatic system. Discover the latest papers using imaging techniques to track CSF outflow into the lymphatic system in animal models.

© 2022 Meta ULC. All rights reserved