Differential Proteome Analysis of Human Neuroblastoma Xenograft Primary Tumors and Matched Spontaneous Distant Metastases

Scientific Reports
Lorena HänelTobias Lange

Abstract

Metastasis formation is the major cause for cancer-related deaths and the underlying mechanisms remain poorly understood. In this study we describe spontaneous metastasis xenograft mouse models of human neuroblastoma used for unbiased identification of metastasis-related proteins by applying an infrared laser (IR) for sampling primary tumor and metastatic tissues, followed by mass spectrometric proteome analysis. IR aerosol samples were obtained from ovarian and liver metastases, which were indicated by bioluminescence imaging (BLI), and matched subcutaneous primary tumors. Corresponding histology proved the human origin of metastatic lesions. Ovarian metastases were commonly larger than liver metastases indicating differential outgrowth capacities. Among ~1,900 proteins identified at each of the three sites, 55 proteins were differentially regulated in ovarian metastases while 312 proteins were regulated in liver metastases. There was an overlap of 21 and 7 proteins up- and down-regulated at both metastatic sites, respectively, most of which were so far not related to metastasis such as LYPLA2, EIF4B, DPY30, LGALS7, PRPH, and NEFM. Moreover, we established in vitro sublines from primary tumor and metastases and demonstrate dif...Continue Reading

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Citations

Feb 26, 2020·International Journal of Molecular Sciences·Pascal SteffenHartmut Schlüter
Dec 31, 2021·Cancer Metastasis Reviews·Wendy E Bindeman, Barbara Fingleton

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Datasets Mentioned

BETA
PXD009744

Methods Mentioned

BETA
xenograft
dissection
bioluminescence imaging
FCS
bichinonic acid assay
acetylation
flow cytometry
light microscopy
profiler
glycosylation

Software Mentioned

Andromeda
Perseus
Pannoramic viewer
Max Quant
MaxQuant

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