Differential proteomic analysis of endometrial fluid suggests increased inflammation and impaired glucose metabolism in non-implantative IVF cycles and pinpoints PYGB as a putative implantation marker

Human Reproduction
Mikel AzkargortaRoberto Matorras

Abstract

Is there any difference in the protein composition of the endometrial fluid aspirate (EFA) obtained the day of embryo transfer in in vitro fertilization (IVF) cycles achieving and not achieving pregnancy? Comparative analysis identified a differential protein expression pattern in 'implantative' and 'non-implantative' IVF cycles. EFA allows non-invasive characterization of the endometrium, and may contain important information on its receptivity when performing (IVF) cycles. Endometrial side of implantation has usually been studied with endometrial biopsy in an IVF cycle prior to embryo transfer, focusing on 'receptive/non-receptive' endometria and with low-throughput proteomic techniques. We have compared the protein expression patterns in EFA from a total of 110 women undergoing IVF, corresponding to 50 implantative and 60 non-implantative IVF cycles. Discovery (38 patients) and Validation (42 patients) sample cohorts were analyzed using a high-throughput differential proteomic approach. Then, the differential expression of glycogen phosphorylase B (PYGB) was validated by western blotting in an additional cohort (30 patients). The study period was 18 months. The population under study consisted of 110 women aged 18-40 years o...Continue Reading

Citations

Feb 26, 2020·Human Reproduction Update·Purificación Hernández-VargasFrancisco Domínguez
May 30, 2020·Human Reproduction Update·Sophie BrouilletSamir Hamamah
Aug 29, 2020·Biology of Reproduction·Xi GuoXiaoyan Chen
May 29, 2020·Technology in Cancer Research & Treatment·Boning XiaChang Liu
Aug 28, 2020·International Journal of Molecular Sciences·Chiara LuongoFrancisco Alberto García-Vázquez
Dec 23, 2019·Journal of Assisted Reproduction and Genetics·Roberto MatorrasCristina Alonso
Jun 16, 2021·Systems Biology in Reproductive Medicine·Roberto MatorrasTeresa Sardon
Jul 6, 2021·Infectious Diseases in Obstetrics and Gynecology·Marta Bregón-VillahozRoberto Matorras
Aug 28, 2021·Animal Reproduction Science·Kassandra SandovalMatthew Dean
Jan 16, 2022·Clinical Reviews in Allergy & Immunology·Alice ZavattaIrene Cetin

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