Abstract
The comparative study of differentially expression of protein profiles of hepatocellular carcinoma cell lines with various metastasic potential and screening key molecules related to hepatocellular carcinoma metastasis and recurrence. Using two-dimensional electrophoresis and liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS), we analyzed differentially displayed proteomics of human hepatocellular carcinoma cell lines Hep3B, MHCC97L, MHCC97H with different metastasic potential. Approximate 1,000 protein spots were detected on silver-stained gel by ImageMaster (977+/-113 spots in Hep3B, 1092+/-40 in MHCC97L, and 889+/-14 in MHCC97H). Fifty distinct different protein spots were analyzed with online LC-ESI-MS/MS. Only 26 protein spots had a positive result, including annexin1, S100A4, and so on. In comparison with nonmetastasis Hep3B cell lines, there were 16 proteins overexpressed in MHCC97H and MHCC97L, 10 proteins underexpressed in MHCC97H and MHCC97L. Applying cell immunohistochemistry and RT-PCR, we further validated two interesting and different proteins, annexin1 and S100A4. The protein profile of metastatic hepatocellular carcinoma cell lines displayed obvious differences compared with no...Continue Reading
Citations
Apr 27, 2005·Brain Research. Brain Research Reviews·Lap HoGiulio Maria Pasinetti
Jun 27, 2006·Oncogene·J T FengL Beretta
Mar 18, 2006·BMC Cancer·Timothy M PawlikHenry M Kuerer
Nov 2, 2005·Journal of Cancer Research and Clinical Oncology·Hai-Yan SongZhao-You Tang
Jun 4, 2014·Mass Spectrometry Reviews·Wenna NieHaitao Lu
Oct 28, 2008·Biochimica Et Biophysica Acta·Fabien RondepierreFrançoise Degoul
Nov 13, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Rajagopal N AravalliErik N K Cressman
Aug 13, 2011·Journal of Surgical Oncology·Yoon-Goo KangChang-Suk Kang
May 17, 2005·Journal of Hepatology·Romain Parent, Laura Beretta
Apr 26, 2006·Proteomics·Da-Qiang LiZhi-Min Shao
Oct 20, 2006·Proteomics·Jie Feng CuiPeng Yuan Yang
Oct 28, 2006·Proteomics·Zhi DaiZhao-You Tang
Mar 5, 2008·Proteomics·Yuan LiYinkun Liu
May 19, 2005·British Journal of Cancer·D M HelfmanM Grigorian
Dec 24, 2005·Proteomics·Huali ShenPengyuan Yang
Jul 6, 2014·American Journal of Physiology. Gastrointestinal and Liver Physiology·Huaidong HuHong Ren
Apr 7, 2011·Molecular BioSystems·Yanyan YuPengyuan Yang
Oct 28, 2020·Technology in Cancer Research & Treatment·Eda G Ramirez-VallesCarlos A Alba-Fierro
Jul 14, 2007·Molecular & Cellular Proteomics : MCP·Wei SunFuchu He
Jun 7, 2007·Journal of Cell Science·Pai-Sheng ChenMin-Liang Kuo
May 27, 2021·Signal Transduction and Targeted Therapy·Haoting SunLunxiu Qin