Differential Proteomic Analysis Predicts Appropriate Applications for the Secretome of Adipose-Derived Mesenchymal Stem/Stromal Cells and Dermal Fibroblasts

Stem Cells International
Stefania NiadaAnna Teresa Brini

Abstract

The adult stem cell secretome is currently under investigation as an alternative to cell-based therapy in regenerative medicine, thanks to the remarkable translational opportunity and the advantages in terms of handling and safety. In this perspective, we recently demonstrated the efficient performance of the adipose-derived mesenchymal stem/stromal cell (ASC) secretome in contrasting neuroinflammation in a murine model of diabetic neuropathy, where the administration of factors released by dermal fibroblasts (DFs) did not exert any effect. Up to now, the complex mixture of the constituents of the conditioned medium from ASCs has not been fully deepened, although its appropriate characterization is required in the perspective of a clinical use. Herein, we propose the differential proteomic approach for the identification of the players accounting for the functional effects of the cell secretome with the aim to unravel its appropriate applications. Out of 967 quantified proteins, 34 and 62 factors were found preponderantly or exclusively secreted by ASCs and DFs, respectively. This approach led to the recognition of distinct functions related to the conditioned medium of ASCs and DFs, with the former being involved in the regula...Continue Reading

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Citations

Jan 2, 2019·Bioengineering·Andrea da Fonseca Ferreira, Dawidson Assis Gomes
Jan 18, 2019·Expert Opinion on Biological Therapy·Mario Rivera-IzquierdoConsolación Melguizo
Jun 11, 2020·International Journal of Molecular Sciences·Svetlana MiroshnichenkoAnastasiya Solovieva
Feb 23, 2020·International Journal of Molecular Sciences·Loubna MaziniGabriel Malka
Nov 18, 2020·Regenerative Medicine·Cristiana MarcozziRosalba Gornati
Feb 13, 2021·Journal of Personalized Medicine·Loubna MaziniGabriel Malka
Dec 5, 2020·Stem Cell Research & Therapy·Chiara GiannasiAnna Teresa Brini
May 1, 2021·Frontiers in Bioengineering and Biotechnology·Cristiano CarlomagnoAnna Teresa Brini
Sep 4, 2021·3 Biotech·Amita Ajit, Indu Ambika Gopalankutty

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Methods Mentioned

BETA
chips
Protein Assay

Software Mentioned

ChemiDoc Imaging
STRING
MeV
Image [UNK]
Mascot
GraphPad Prism
MaxQuant

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