Differential proteomic profiling identifies novel molecular targets of pterostilbene against experimental diabetes

Journal of Cellular Physiology
Sireesh DornadulaRamkumar K Mohanram

Abstract

Pterostilbene (PTS), a naturally occurring stilbene, confers protection against oxidative and cytokine stress induced pancreatic β-cell apoptosis in vitro and in vivo. To provide insights into the molecular mechanism, we performed a proteomic study on the pancreas of PTS-treated diabetic mice using electrospray ionization tandem-mass spectrometry (LC-MS/MS). A total of 1,260 proteins were detected in triplicate samples. Of which, 359 proteins were found to be differentially regulated in streptozotocin-induced diabetic mice pancreas with two fold difference ( P < 0.05, two or more peptides) and on PTS treatment 315 proteins were normalized to control levels. Gene ontology (GO) indicated that majority of the differentially regulated proteins are involved in cellular functions such as metabolism, cellular structure, oxidative stress, endoplasmic-reticulum-associated protein degradation (ERAD) pathway and several stress sensors. Protein-protein interaction network analysis of these differentially expressed proteins showed clustering of proteins involved in protein processing in endoplasmic reticulum (protein synthesis machinery and protein folding), oxidative phosphorylation/oxidative stress proteins, oligosaccharide metabolic proc...Continue Reading

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Citations

Apr 1, 2021·Free Radical Research·Karan Naresh AminKunka Mohanram Ramkumar
Apr 27, 2021·Oxidative Medicine and Cellular Longevity·Karan Naresh AminRamkumar Kunka Mohanram
Jul 14, 2021·Experimental Gerontology·Goutham V Ganesh, Kunka Mohanram Ramkumar
Oct 28, 2019·Journal of Proteome Research·Atef Mannaa, Franz-Georg Hanisch
Oct 31, 2019·Current Medicinal Chemistry·Saioa Gómez-ZoritaMaría P Portillo

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