Differential proteomic response to heat stress in thermal Agrostis scabra and heat-sensitive Agrostis stolonifera

Physiologia Plantarum
Chenping Xu, Bingru Huang

Abstract

Knowledge of heat-responsive proteins is critical for further understanding of the molecular mechanisms of heat tolerance. The objective of this study was to compare proteins differentially expressed in two C(3) grass species contrasting in heat tolerance, heat-tolerant thermal Agrostis scabra and heat-sensitive Agrostis stolonifera L., and to identify heat-responsive proteins for short- and long-term responses. Plants were exposed to 20/15 degrees C (day/night, control) or 40/35 degrees C (day/night, heat stress) in growth chambers. Leaves were harvested at 2 and 10 days after temperature treatment. Proteins were extracted and separated by fluorescence difference gel electrophoresis (DIGE). Thermal A. scabra had superior heat tolerance than A. stolonifera, as indicated by the maintenance of higher chlorophyll content and photochemical efficiency under heat stress. The two-dimensional difference electrophoresis detected 68 heat-responsive proteins in the two species. Thermal A. scabra had more protein spots either down- or up-regulated at 2 days of heat stress, but fewer protein spots were altered at 10 days of heat stress compared with A. stolonifera. Many protein spots exhibited transient down-regulation in thermal A. scabra ...Continue Reading

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Sep 1, 2012·Journal of Experimental Botany·Yin An YaoGang Xu
Jun 4, 2013·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Peng ZhangTaiwu Li
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Jul 27, 2017·Frontiers in Plant Science·David JespersenBingru Huang

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