PMID: 6107034May 1, 1980Paper

Differential pulse-polarographic analysis of tobacco mosaic virus

Acta Virologica
G Ruttkay-Nedecký, E Palecek

Abstract

The tobacco mosaic virus (TMV) strain vulgare and its mutant TMV 483 (with glutamine-9 replaced by histidine) and the denatured protein of TMV vulgare were analysed by direct current (d. c.) and differential (derivative) pulse polarography (DPP) in the basic electrolyte composed of 0.001 M Co(NH3)6Cl3, 0.1 M NH4Cl and 0.1 M NH3 at 0 degrees C. The DPP method gave a substantially better resolution of the polarographic catalytic maxima A and B, but a much lower resolution of the maxima B and C, as compared with the d. c. polarographic method. The clear differentiation of the maximum A from maximum B by DPP permitted to study the variation of maximum A in the course of alkaline degradation of TMV. But for the study of TMV protein denaturation the d. c. polarography is preferable, because the denaturation is accompanied by the appearance and rise of maximum C which can be clearly differentiated from maximum B by d. c. polarography rather than by DPP. The DPP method was more, sensitive than d. c. polarography. The denatured TMV protein can be determined by DPP at concentrations around 0.1 microgram/ml.

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

International Journal of Environmental Analytical Chemistry
M E MohamedH Y Aboul-Enein
Journal of Pharmaceutical and Biomedical Analysis
A Snycerski
© 2021 Meta ULC. All rights reserved