PMID: 9429764Jan 16, 1998Paper

Differential purinergic receptor signalling in osteoclasts and osteoblastic cells

Cellular Signalling
L C LuoJ Ferrier

Abstract

There are differences between osteoclasts and osteoblastic cells in their cytosolic calcium responses to purinergic receptor activation. Application of 50 or 100 microM extracellular ATP inhibits the calcium response to a second application of ATP in osteoblastic rat osteosarcoma UMR 106 cells, but not in rabbit osteoclasts. This shows that there is adaptation to the extracellular, ATP in osteoclasts, but not in the UMR 106 cells. Extracellular washing of the UMR 106 cells restores the calcium response to ATP partially but not completely, indicating that there is a purinergic receptor activation-induced desensitisation of the receptor or its linked signalling pathways. In contrast to these results, if extracellular UTP is applied first, application of ATP produces no calcium response in osteoclasts, with or without washing, while in the UMR 106 cells there is some response to the ATP, which is greatly enhanced by washing. This indicates that UTP induces a complete desensitisation of the purinergic receptor/calcium signalling system in osteoclasts, but not in the osteoblastic cells, in which there is simply competition between UTP and ATP for the same receptors. Taken together, these results demonstrate that ATP and UTP could di...Continue Reading

References

Aug 14, 1992·Biochemical and Biophysical Research Communications·S L Xia, J Ferrier
May 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·C SchöflJ A Gallagher
Jul 1, 1991·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·H KumagaiC R Filburn
Nov 1, 1994·Cellular Signalling·H Yu, J Ferrier
Jan 1, 1994·Pharmacology & Therapeutics·M P Abbracchio, G Burnstock
Sep 1, 1993·The American Journal of Physiology·G R Dubyak, C el-Moatassim
Jan 28, 1994·Cell·T KishimotoS Akira
Mar 15, 1993·Biochemical and Biophysical Research Communications·H Yu, J Ferrier

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Citations

Nov 11, 2005·APMIS. Supplementum·Niklas Rye Jørgensen
May 30, 2009·The Tohoku Journal of Experimental Medicine·Hanbyoul ChoJae-Hoon Kim
Mar 21, 2002·Expert Opinion on Therapeutic Targets·J H TobiasA M Scutt
Dec 13, 2005·Cell Biochemistry and Function·Taisuke KonoKoh-ichi Enomoto

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