Differential Ratios of Omega Fatty Acids (AA/EPA+DHA) Modulate Growth, Lipid Peroxidation and Expression of Tumor Regulatory MARBPs in Breast Cancer Cell Lines MCF7 and MDA-MB-231

PloS One
Prakash P MansaraRuchika Kaul-Ghanekar

Abstract

Omega 3 (n3) and Omega 6 (n6) polyunsaturated fatty acids (PUFAs) have been reported to exhibit opposing roles in cancer progression. Our objective was to determine whether different ratios of n6/n3 (AA/EPA+DHA) FAs could modulate the cell viability, lipid peroxidation, total cellular fatty acid composition and expression of tumor regulatory Matrix Attachment Region binding proteins (MARBPs) in breast cancer cell lines and in non-cancerous, MCF10A cells. Low ratios of n6/n3 (1:2.5, 1:4, 1:5, 1:10) FA decreased the viability and growth of MDA-MB-231 and MCF7 significantly compared to the non-cancerous cells (MCF10A). Contrarily, higher n6/n3 FA (2.5:1, 4:1, 5:1, 10:1) decreased the survival of both the cancerous and non-cancerous cell types. Lower ratios of n6/n3 selectively induced LPO in the breast cancer cells whereas the higher ratios induced in both cancerous and non-cancerous cell types. Interestingly, compared to higher n6/n3 FA ratios, lower ratios increased the expression of tumor suppressor MARBP, SMAR1 and decreased the expression of tumor activator Cux/CDP in both breast cancer and non-cancerous, MCF10A cells. Low n6/n3 FAs significantly increased SMAR1 expression which resulted into activation of p21WAF1/CIP1 in MDA...Continue Reading

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Citations

Jul 8, 2008·The Journal of Nutritional Biochemistry·Patricia D BiondoCatherine J Field
Sep 1, 2016·BMC Complementary and Alternative Medicine·Abilasha Gayani JayathilakeXiao Qun Su
Mar 4, 2017·Materials Science & Engineering. C, Materials for Biological Applications·Mailine GehrckeLetícia Cruz
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Jun 27, 2019·Breast Cancer Research and Treatment·Marnie NewellCatherine J Field
Jul 3, 2021·International Journal of Molecular Sciences·Kamila P LiputMariusz Pierzchała

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Methods Mentioned

BETA
X-ray

Software Mentioned

GraphPad prism
ImageJ
ImageJ Image Processing and Analysis

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