Differential recognition of citrate and a metal-citrate complex by the bacterial chemoreceptor Tcp

The Journal of Biological Chemistry
T IwamaIkuro Kawagishi

Abstract

The chemoreceptor Tcp of Salmonella enterica serovar Typhimurium can sense citrate and a metal-citrate complex as distinct attractants. In this study, we tried to investigate the molecular mechanism of this discrimination. That citrate binds directly to Tcp was verified by the site-specific thiol modification assays using membrane fractions prepared from Escherichia coli cells expressing the mutant Tcp receptors in which single Cys residues were introduced at positions in the putative ligand-binding pocket. To determine the region responsible for the ligand discrimination, we screened for mutations defective in taxis to magnesium in the presence of citrate. All of the isolated mutants from random mutagenesis with hydroxylamine were defective in both citrate and metal-citrate sensing, and the mutated residues are located in or near the alpha1-alpha2 and alpha3-alpha4 loops within the periplasmic domain. Further analyses with site-directed replacements around these regions demonstrated that the residue Asn(67), which is presumed to lie at the subunit interface of the Tcp homodimer, plays a critical role in the recognition of the metal-citrate complex but not that of citrate. Various amino acids at this position differentially aff...Continue Reading

References

Dec 1, 1979·Journal of Bacteriology·T D Ingolia, D E Koshland
Aug 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·M S SpringerJ Adler
Oct 1, 1979·Journal of Bacteriology·M Kihara, R M Macnab
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A J Wolfe, H C Berg
Oct 1, 1988·Journal of Bacteriology·C Wolff, J S Parkinson
May 1, 1987·Journal of Bacteriology·A J WolfeH C Berg
Jun 4, 1993·Cell·J S Parkinson
Apr 1, 1993·Protein Science : a Publication of the Protein Society·C J Jeffery, D E Koshland
Jan 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·K Yamamoto, Y Imae
Dec 1, 1996·Current Opinion in Structural Biology·D E Koshland
Feb 5, 2000·Current Biology : CB·J Stock, M Levit
Jul 14, 2001·Advances in Microbial Physiology·I B Zhulin
Nov 13, 2004·Trends in Microbiology·Victor Sourjik
Dec 2, 2004·Nature Reviews. Molecular Cell Biology·George H Wadhams, Judith P Armitage
Apr 2, 2005·Current Opinion in Microbiology·John S ParkinsonClaudia A Studdert

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Citations

Nov 13, 2010·Metallomics : Integrated Biometal Science·Joshua J LensbouerRobert P Doyle
Apr 17, 2012·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·J A GaviraE Pineda-Molina
Dec 13, 2006·Microbiology and Molecular Biology Reviews : MMBR·Thorsten MascherGottfried Unden
Jan 19, 2007·Molecular Microbiology·J Christian Perez, Eduardo A Groisman
Mar 2, 2011·Journal of Molecular Recognition : JMR·Jesús LacalTino Krell
Oct 16, 2015·Environmental Microbiology·David Martín-MoraTino Krell
Aug 19, 2015·Frontiers in Microbiology·Carla SolórzanoCristina Madrid
Jun 7, 2015·Applied and Environmental Microbiology·Vanina GarcíaTino Krell
Oct 27, 2017·Microbiology and Molecular Biology Reviews : MMBR·Álvaro OrtegaTino Krell

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